PMID- 8853727
OWN - NLM
STAT- MEDLINE
DCOM- 19961231
LR  - 20190718
IS  - 0269-9370 (Print)
IS  - 0269-9370 (Linking)
VI  - 10
IP  - 9
DP  - 1996 Aug
TI  - Polymerase chain reaction on cerebrospinal fluid for diagnosis of 
      virus-associated opportunistic diseases of the central nervous system in 
      HIV-infected patients.
PG  - 951-8
AB  - OBJECTIVE: To assess the diagnostic reliability of polymerase chain reaction 
      (PCR) on cerebrospinal fluid (CSF) for virus-associated opportunistic diseases of 
      the central nervous system (CNS) in HIV-infected patients. DESIGN: CSF samples 
      from 500 patients with HIV infection and CNS symptoms were examined by PCR. In 
      219 patients the PCR results were compared with CNS histological findings. 
      METHODS: Nested PCR for detection of herpes simplex virus (HSV) type 1 or 2, 
      varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), 
      human herpesvirus 6 (HHV-6), and JC virus (JCV) DNA. Histopathological 
      examination of CNS tissue obtained at autopsy or on brain biopsy. RESULTS: DNA of 
      one or more viruses was found in CSF in 181 out of 500 patients (36%; HSV-1 2%, 
      HSV-2 1%, VZV 3%, CMV 16%, EBV 12%, HHV-6 2%, and JCV 9%). Among the 219 patients 
      with histological CNS examination, HSV-1 or 2 was detected in CSF in all six 
      patients (100%) with HSV infection of the CNS, CMV in 37 out of 45 (82%) with CMV 
      infection of the CNS, EBV in 35 out of 36 (97%) with primary CNS lymphoma, JCV in 
      28 out of 39 (72%) with progressive multifocal leukoencephalopathy. Furthermore, 
      HSV-1 was found in one, VZV in four, CMV in three, EBV in three, HHV-6 in seven, 
      and JCV in one patient without histological evidence of the corresponding CNS 
      disease. CONCLUSIONS: CSF PCR has great relevance for diagnosis of virus-related 
      opportunistic CNS diseases in HIV-infected patients as demonstrated by its high 
      sensitivity, specificity, and the frequency of positive findings.
FAU - Cinque, P
AU  - Cinque P
AD  - Department of Infectious Diseases, University of Milan, Italy.
FAU - Vago, L
AU  - Vago L
FAU - Dahl, H
AU  - Dahl H
FAU - Brytting, M
AU  - Brytting M
FAU - Terreni, M R
AU  - Terreni MR
FAU - Fornara, C
AU  - Fornara C
FAU - Racca, S
AU  - Racca S
FAU - Castagna, A
AU  - Castagna A
FAU - Monforte, A D
AU  - Monforte AD
FAU - Wahren, B
AU  - Wahren B
FAU - Lazzarin, A
AU  - Lazzarin A
FAU - Linde, A
AU  - Linde A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/cerebrospinal fluid/*diagnosis
MH  - Brain Diseases/cerebrospinal fluid/*diagnosis/etiology/virology
MH  - Cytomegalovirus/genetics
MH  - DNA Primers
MH  - DNA, Viral/*cerebrospinal fluid
MH  - HIV Infections/*complications
MH  - HIV-1/*genetics
MH  - Herpesviridae/genetics
MH  - Herpesvirus 4, Human/genetics
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1097/00002030-199610090-00004 [doi]
PST - ppublish
SO  - AIDS. 1996 Aug;10(9):951-8. doi: 10.1097/00002030-199610090-00004.