PMID- 8854879
OWN - NLM
STAT- MEDLINE
DCOM- 19970218
LR  - 20071115
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 105
IP  - 4
DP  - 1996 Oct
TI  - Aneuploid epididymal sperm detected in chromosomally normal and Robertsonian 
      translocation-bearing mice using a new three-chromosome FISH method.
PG  - 204-10
AB  - We present a new method to detect epididymal sperm aneuploidy (ESA) in mice using 
      simultaneous fluorescence in situ hybridization (FISH) with DNA probes specific 
      for mouse chromosomes X, Y and 8. The method was applied to Robertsonian (Rb) 
      translocation (8.14) heterozygotes and homozygotes as well as the chromosomally 
      normal B6C3F1. The sex ratios of sperm did not differ from the expected 1:1 and 
      the hybridization efficiencies were approximately 99.7% for over 60 000 sperm 
      analyzed. Mice heterozygous for Rb (8.14) produced about tenfold higher rates of 
      sperm with chromosome 8 hyperhaploidy than did Rb (8.14) homozygotes or 
      chromosomally normal mice, while frequencies of sperm with hyperhaploidies for 
      chromosomes X and Y were unaffected in all three lines of mice. Hyperhaploid 
      frequencies obtained with the ESA method were consistent with those of the 
      previous testicular FISH method and were validated by published data obtained by 
      conventional cytogenetic analyses (meiotic metaphase II and first cleavage). 
      Thus, the mouse three-chromosome ESA assay together with the previously developed 
      aneuploidy assay for human sperm constitute a promising pair of interspecific 
      biomarkers for comparative studies of the genetic and physiologic mechanisms of 
      the induction and persistence of aneuploidy in male germ cells.
FAU - Lowe, X
AU  - Lowe X
AD  - Biology and Biotechnology Research Program, L-452, P. O. Box 808, 7000 East 
      Avenue, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA. 
      wyrobek1@llnl.gov
FAU - O'Hogan, S
AU  - O'Hogan S
FAU - Moore, D 2nd
AU  - Moore D 2nd
FAU - Bishop, J
AU  - Bishop J
FAU - Wyrobek, A
AU  - Wyrobek A
LA  - eng
GR  - IAG Y01-ES-10203-00/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Cytogenetics/methods
MH  - Epididymis/*cytology
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Heterozygote
MH  - Homozygote
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Spermatozoa/*physiology
MH  - *Translocation, Genetic
EDAT- 1996/10/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
PST - ppublish
SO  - Chromosoma. 1996 Oct;105(4):204-10.