PMID- 8855250
OWN - NLM
STAT- MEDLINE
DCOM- 19961125
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 20
DP  - 1996 Oct 1
TI  - Moderate increase in histone acetylation activates the mouse mammary tumor virus 
      promoter and remodels its nucleosome structure.
PG  - 10741-6
AB  - The mouse mammary tumor virus (MMTV) promoter is regulated by steroid hormones 
      through a hormone-responsive region that is organized in a positioned nucleosome. 
      Hormone induction leads to a structural change of this nucleosome which makes its 
      DNA more sensitive to cleavage by DNase I and enables simultaneous binding of all 
      relevant transcription factors. In cells carrying either episomal or 
      chromosomally integrated MMTV promoters, moderate acetylation of core histones, 
      generated by treatment with low concentrations of the histone deacetylase 
      inhibitors sodium butyrate or trichostatin A, enhances transcription from the 
      MMTV promoter in the absence of hormone and potentiates transactivation by either 
      glucocorticoids or progestins. At higher concentrations, histone deacetylase 
      inhibitors reduce basal and hormone induced MMTV transcription. Inducing 
      inhibitor concentrations lead to the same type of nucleosomal DNase I 
      hypersensitivity as hormone treatment, suggesting that moderate acetylation of 
      core histone activates the MMTV promoter by mechanisms involving chromatin 
      remodeling similar to that generated by the inducing hormones.
FAU - Bartsch, J
AU  - Bartsch J
AD  - Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany.
FAU - Truss, M
AU  - Truss M
FAU - Bode, J
AU  - Bode J
FAU - Beato, M
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Chromatin)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glucocorticoids)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Histones)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (Nucleosomes)
RN  - 3X2S926L3Z (trichostatin A)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Cell Cycle
MH  - Cell Line
MH  - Chromatin/ultrastructure
MH  - Deoxyribonuclease I/metabolism
MH  - Dexamethasone/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - *Gene Expression Regulation, Viral
MH  - Glucocorticoids/pharmacology
MH  - Histone Deacetylase Inhibitors
MH  - Histones/*physiology
MH  - Hydroxamic Acids/pharmacology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Nucleosomes/*physiology/ultrastructure
MH  - Plasmids
MH  - *Promoter Regions, Genetic
MH  - Transcription, Genetic
PMC - PMC38225
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
PMCR- 1997/04/01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
PHST- 1997/04/01 00:00 [pmc-release]
AID - 10.1073/pnas.93.20.10741 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10741-6. doi: 
      10.1073/pnas.93.20.10741.