PMID- 8856246
OWN - NLM
STAT- MEDLINE
DCOM- 19970103
LR  - 20180215
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 73
IP  - 4
DP  - 1996
TI  - Morphine modulates migration of monocytes.
PG  - 526-31
AB  - Macrophages seem to play an important role in the development of 
      glomerulosclerosis. In the present study we evaluated the effect of morphine, an 
      active metabolite of heroin, on the migration of monocytes across a filter in a 
      modified Boyden chamber. Morphine-mesangial cell interaction enhanced (p < 0.004) 
      the migration of monocytes across the filter (control 14.2 +/- 0.6 vs. morphine 
      22.1 +/- 1.7 monocytes/HPF). Dimethylthiourea (DMTU), a free radical scavenger, 
      attenuated this effect of morphine. Morphine-monocyte secretory products (MMSP) 
      did not modulate the migration of monocytes. However, the products of interaction 
      between mesangial cells and MMSP enhanced (p < 0.001) the passage of monocytes 
      across the filter. Mesangial cells treated with MMSP showed mRNA expression for 
      monocyte chemoat-tractant peptide-1 (MCP-1). Superoxide also induced mRNA 
      expression for MCP-1 on MC. DMTU attenuated this effect of superoxide. Since 
      morphine activates MC to produce superoxide and DMTU attenuated the effect of 
      superoxide on MC, the effect of morphine on the migration of macrophages may be 
      mediated through superoxide-induced generation of MCP-1. We conclude that 
      morphine enhances the migration of monocytes. This effect of morphine may be 
      contributing to the development of glomerulosclerosis in patients with heroin 
      addiction.
FAU - Singhal, P C
AU  - Singhal PC
AD  - Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, N.Y. 
      11040, USA.
FAU - Shan, Z
AU  - Shan Z
FAU - Garg, P
AU  - Garg P
FAU - Sharma, K
AU  - Sharma K
FAU - Sharma, P
AU  - Sharma P
FAU - Gibbons, N
AU  - Gibbons N
LA  - eng
GR  - 2RO1 DA06753/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Narcotics)
RN  - 0 (RNA, Messenger)
RN  - 11062-77-4 (Superoxides)
RN  - 76I7G6D29C (Morphine)
RN  - 8P30PMD17W (1,3-dimethylthiourea)
RN  - GYV9AM2QAG (Thiourea)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Communication/drug effects
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Chemokine CCL2/biosynthesis/pharmacology
MH  - Culture Media, Conditioned
MH  - Free Radical Scavengers/pharmacology
MH  - Glomerular Mesangium/cytology/drug effects/ultrastructure
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Monocytes/*drug effects/metabolism/ultrastructure
MH  - Morphine/*pharmacology
MH  - Narcotics/*pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Superoxides/metabolism
MH  - Thiourea/analogs & derivatives/pharmacology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000189135 [doi]
PST - ppublish
SO  - Nephron. 1996;73(4):526-31. doi: 10.1159/000189135.