PMID- 8856699
OWN - NLM
STAT- MEDLINE
DCOM- 19970306
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 7
IP  - 9
DP  - 1996 Jun 17
TI  - An ICE inhibitor, z-VAD-DCB attenuates ischaemic brain damage in the rat.
PG  - 1465-8
AB  - Interleukin-1 beta (IL-1 beta) converting enzyme (ICE) cleaves pro-IL-1 beta to 
      produce mature IL-beta, and is a member of a family of proteases implicated in 
      apoptosis. Intracerebroventricular (i.c.v.) administration of an irreversible ICE 
      inhibitor, z-VAD-DCB (1 pmol, 30 min before and 15 min, 2, 4, 6 and 8 h after 
      surgery) markedly reduced (50 +/- 4%, p < 0.001) infarct volume measured 24 h 
      after focal cerebral ischaemia (middle cerebral artery occlusion, MCAo) in the 
      rat. Inhibition of damage was observed in the cortex (51 +/- 5% reduction) and 
      striatum (42 +/- 6% reduction). These data implicate ICE in ischaemic neuronal 
      death in vivo. Inhibition of ICE could reduce ischaemic damage either by 
      preventing IL-1 beta synthesis or by inhibiting apoptosis or by both of these 
      processes, and may provide a useful therapeutic approach to the inhibition of 
      ischaemic brain damage.
FAU - Loddick, S A
AU  - Loddick SA
AD  - School of Biological Sciences, University of Manchester, UK.
FAU - MacKenzie, A
AU  - MacKenzie A
FAU - Rothwell, N J
AU  - Rothwell NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Interleukin-1)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Oligopeptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (z-VAD-DCB)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
EIN - Neuroreport 1999 Jun 23;10(9):inside back cov
MH  - Animals
MH  - Caspase 1
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cysteine Endopeptidases/*drug effects
MH  - Cysteine Proteinase Inhibitors/*therapeutic use
MH  - Enzyme Activation
MH  - Interleukin-1/*metabolism
MH  - Ischemic Attack, Transient/*prevention & control
MH  - Male
MH  - Molecular Weight
MH  - Monocytes/drug effects/metabolism
MH  - Neurons/*drug effects/pathology
MH  - Neuroprotective Agents/*therapeutic use
MH  - Oligopeptides/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Proteins/antagonists & inhibitors
EDAT- 1996/06/17 00:00
MHDA- 1996/06/17 00:01
CRDT- 1996/06/17 00:00
PHST- 1996/06/17 00:00 [pubmed]
PHST- 1996/06/17 00:01 [medline]
PHST- 1996/06/17 00:00 [entrez]
AID - 10.1097/00001756-199606170-00004 [doi]
PST - ppublish
SO  - Neuroreport. 1996 Jun 17;7(9):1465-8. doi: 10.1097/00001756-199606170-00004.