PMID- 8859896
OWN - NLM
STAT- MEDLINE
DCOM- 19970411
LR  - 20220223
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 208
IP  - 2
DP  - 1996 Apr 19
TI  - Intracarotid tumor necrosis factor-alpha administration increases the blood-brain 
      barrier permeability in cerebral cortex of the newborn pig: quantitative aspects 
      of double-labelling studies and confocal laser scanning analysis.
PG  - 85-8
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays a crucial role in the pathogenesis 
      of the central nervous system infections. The aim of the present study was to 
      analyze quantitatively the changes in the blood-brain barrier (BBB) permeability 
      after the intracarotid injection of TNF-alpha. Recombinant human TNF-alpha was 
      injected into the left internal carotid artery of anesthetized newborn pigs (n = 
      48) in the doses of 0, 1000, 10 000 and 100 000 IU, respectively. Before, as well 
      as 1, 2, 4, 8, and 16 h after the challenge, the extravasation of a small (sodium 
      fluorescein (SF), mw 376), and a large (Evan's blue-albumin (EBA), mw 67 000) 
      tracer was determined concomitantly by spectrophotometry in the cerebral cortex 
      of the animals. There was a time- and dose-dependent increase in BBB permeability 
      both for SF and EBA; however, significant (P < 0.05) BBB opening for albumin only 
      developed 2 h after the challenge. In the morphological study the same excitable 
      tracers, identical experimental protocol and groups were used. Cryostat sections 
      of brain tissue were viewed for optical sectioning with a confocal laser scanning 
      microscope equipped with an argon/krypton ion laser. A diffuse BBB opening for SF 
      and a moderate perivascular extravasation for EBA were found in the cortices of 
      TNF-alpha-treated animals. We conclude that significant increases in 
      intravascular TNF-alpha-concentration during neonatal infections may result in 
      vasogenic brain edema formation.
FAU - Abraham, C S
AU  - Abraham CS
AD  - Department of Pediatrics, Albert Szent-Gyorgyi Medical University, Szeged, 
      Hungary. abraham@pedia.szote.u-szeged.hu
FAU - Deli, M A
AU  - Deli MA
FAU - Joo, F
AU  - Joo F
FAU - Megyeri, P
AU  - Megyeri P
FAU - Torpier, G
AU  - Torpier G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Albumins)
RN  - 0 (Fluoresceins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 45PG892GO1 (Evans Blue)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Albumins/*pharmacokinetics
MH  - Animals
MH  - Animals, Newborn
MH  - Blood-Brain Barrier/*drug effects
MH  - Carotid Arteries
MH  - Cerebral Cortex/blood supply/metabolism/physiopathology
MH  - Dose-Response Relationship, Drug
MH  - Edema/metabolism/physiopathology
MH  - Evans Blue/*pharmacokinetics
MH  - Female
MH  - Fluorescein
MH  - Fluoresceins/*pharmacokinetics
MH  - Injections, Intra-Arterial
MH  - Lasers
MH  - Male
MH  - Microscopy, Confocal
MH  - Swine
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1996/04/19 00:00
MHDA- 1996/04/19 00:01
CRDT- 1996/04/19 00:00
PHST- 1996/04/19 00:00 [pubmed]
PHST- 1996/04/19 00:01 [medline]
PHST- 1996/04/19 00:00 [entrez]
AID - 0304-3940(96)12546-5 [pii]
AID - 10.1016/0304-3940(96)12546-5 [doi]
PST - ppublish
SO  - Neurosci Lett. 1996 Apr 19;208(2):85-8. doi: 10.1016/0304-3940(96)12546-5.