PMID- 8861647
OWN - NLM
STAT- MEDLINE
DCOM- 19970501
LR  - 20190830
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 49
IP  - 1-2
DP  - 1996 Mar
TI  - Diagnosis of porcine reproductive and respiratory syndrome using infected 
      alveolar macrophages collected from live pigs.
PG  - 105-15
AB  - A highly sensitive method of detecting infection of live pigs with porcine 
      reproductive and respiratory syndrome virus (PRRSV) was developed by testing 
      alveolar macrophages collected by pulmonary lavage. Five pigs were exposed by 
      oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks 
      (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were 
      collected from each pig just before exposure to PRRSV. During the next 9 weeks 
      sera were collected at weekly intervals and alveolar macrophages were collected 
      at weeks 2 and 4-9. Both sera and alveolar macrophages were suitable for 
      detecting early infection, but alveolar macrophages were clearly the better 
      sample after longer intervals. Virus was last isolated from serum at week 4 (from 
      1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 
      pigs at week 4 and from at least 2 pigs at each of the weekly intervals 
      thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most sensitive method 
      of testing alveolar macrophages for PRRSV was cocultivation with MARC-145 cells. 
      None of the pigs had any clinical signs after exposure to PRRSV or as a result of 
      pulmonary lavage and there was no evidence that repeated pulmonary lavage caused 
      anything other than a mild, transient (mild hyperemia) tissue reaction.
FAU - Mengeling, W L
AU  - Mengeling WL
AD  - Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Iowa 50010, USA.
FAU - Vorwald, A C
AU  - Vorwald AC
FAU - Lager, K M
AU  - Lager KM
FAU - Brockmeier, S L
AU  - Brockmeier SL
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
EIN - Vet Microbiol 1996 Oct;52(3-4):317
MH  - Animals
MH  - Bronchoalveolar Lavage/methods/*veterinary
MH  - Bronchoalveolar Lavage Fluid
MH  - Cell Line
MH  - Lung/pathology/virology
MH  - Macrophages, Alveolar/pathology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*diagnosis/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 0378-1135(95)00173-5 [pii]
AID - 10.1016/0378-1135(95)00173-5 [doi]
PST - ppublish
SO  - Vet Microbiol. 1996 Mar;49(1-2):105-15. doi: 10.1016/0378-1135(95)00173-5.