PMID- 8862264
OWN - NLM
STAT- MEDLINE
DCOM- 19961209
LR  - 20061115
IS  - 0148-916X (Print)
IS  - 0148-916X (Linking)
VI  - 64
IP  - 3
DP  - 1996 Sep
TI  - Recognition of lipid antigens by sera of mice infected with Mycobacterium 
      lepraemurium.
PG  - 299-305
AB  - Lipids extracted from mouse tissues infected with Mycobacterium lepraemurium 
      (MLM) were analyzed by thin-layer chromatography. Although the extracted lipids 
      were heterogeneous in polarity, the lipids of intermediate polarity were the ones 
      that predominated. All of the lipids of intermediate polarity were glycosylated 
      species. There were also lipids of low and high polarity, the latter being 
      glycolipids. Compared to lipids extracted from normal tissue (mostly to lipids of 
      high and low polarity), all of the additional lipids extracted from the infected 
      tissue corresponded to lipids present in the purified bacteria. Enzyme-linked 
      immunoassays (ELISAs) were then performed with the whole lipids extracted from 
      purified bacilli, the lipids of high, intermediate and low polarity, and the sera 
      from 20 normal and 20 MLM-infected mice. Lipids of intermediate polarity were 
      specifically recognized by MLM-infected mice. Neither sera (diluted 1:500) from 
      normal mice nor infected mice reacted with the lipids of high or low polarity, 
      but a higher concentration (sera diluted 1:100) of some sera from mice in both 
      groups reacted significantly with these lipids. In the ELISAs the whole-lipid 
      extract and the lipids of intermediate polarity were similarly recognized by the 
      sera of the infected mice. Thus, as observed in human leprosy, the mycobacterial 
      disease in the mouse (murine leprosy) is also accompanied by the development of 
      antibodies to the glycolipids of the infecting microorganism.
FAU - Luna-Herrera, J
AU  - Luna-Herrera J
AD  - Department of Immunology, National School of Biological Sciences, National 
      Polytechnic Institute, Mexico, D.F., Mexico.
FAU - Rojas-Espinosa, O
AU  - Rojas-Espinosa O
FAU - Estrada-Parra, S
AU  - Estrada-Parra S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Lepr Other Mycobact Dis
JT  - International journal of leprosy and other mycobacterial diseases : official 
      organ of the International Leprosy Association
JID - 8505819
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Glycolipids)
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood/*immunology
MH  - Antigens, Bacterial/*chemistry/*immunology
MH  - Chromatography, Thin Layer
MH  - Female
MH  - Glycolipids/immunology/isolation & purification
MH  - Lipids/*immunology/isolation & purification
MH  - Liver/chemistry
MH  - Mice
MH  - Mycobacterium Infections/blood/*immunology
MH  - Mycobacterium lepraemurium/*chemistry/*immunology
MH  - Skin/chemistry/microbiology
MH  - Spleen/chemistry
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PST - ppublish
SO  - Int J Lepr Other Mycobact Dis. 1996 Sep;64(3):299-305.