PMID- 8862448
OWN - NLM
STAT- MEDLINE
DCOM- 19961115
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 70
IP  - 4
DP  - 1996 Oct
TI  - Induction and persistence of cytogenetic damage in mouse splenocytes following 
      whole-body X-irradiation analysed by fluorescence in situ hybridization. II. 
      Micronuclei.
PG  - 375-83
AB  - Micronuclei are formed during cell division either from lagging acentric 
      fragments caused by chromosomal breakage or from whole chromosomes. Female Swiss 
      mice were used to study the induction and persistence of micronuclei (MN) in 
      isolated splenocytes up to 112 days after 2Gy whole-body X-irradiation. 
      Micronucleus frequency in cytokinesis blocked binucleated cells was estimated 
      using acridine orange staining. Fluorescence in situ hybridization (FISH) with 
      murine minor satellite DNA probe was used to detect aneuploidy (MN with a 
      centromere). The initial frequency of micronuclei immediately after X-ray 
      exposure was found to be 39.6 per 100 cells. The MN frequency declined in an 
      exponential manner during the subsequent period and at day 14, reached about half 
      the value observed immediately after irradiation with a further 50% reduction by 
      day 28 and only 3% MN could be detected at day 112 post-irradiation. Some 
      induction of aneuploidy was observed after X-irradiation, deduced in the form of 
      centromere-positive micronuclei (C + MN) as detected by the presence of a minor 
      satellite signal after FISH. Of MN, 23% were centromere-positive in the first 
      fixation time after X-ray exposure. Some of the MN showed two or more centromeric 
      signals. The percent C + MN increased gradually until day 7 post-exposure. At day 
      14, this value started to decline, reaching 13% by 112 days post-irradiation. 
      FISH with a biotinylated minor satellite probe can reliably be used in the 
      cytokinesis blocked micronucleus assay to distinguish the clastogenic and/or 
      aneugenic activity of a test chemical or physical agent.
FAU - Hande, M P
AU  - Hande MP
AD  - Department of Radiation Genetics, Leiden University, The Netherlands.
FAU - Boei, J J
AU  - Boei JJ
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Centromere/radiation effects
MH  - Chromosome Aberrations
MH  - DNA/analysis/radiation effects
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Micronuclei, Chromosome-Defective/*radiation effects
MH  - Spleen/cytology/*radiation effects/ultrastructure
MH  - Whole-Body Irradiation/*adverse effects
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1080/095530096144842 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1996 Oct;70(4):375-83. doi: 10.1080/095530096144842.