PMID- 8862449
OWN - NLM
STAT- MEDLINE
DCOM- 19961115
LR  - 20220408
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 70
IP  - 4
DP  - 1996 Oct
TI  - Chromosome analysis by fluorescence in situ hybridization: further indications 
      for a non-DNA-proportional involvement of single chromosomes in radiation-induced 
      structural aberrations.
PG  - 385-92
AB  - The frequencies of symmetrical complete and incomplete translocations and 
      dicentrics induced in human lymphocytes after in vitro irradiation with 3Gy 
      X-rays were analysed by the use of fluorescence in situ hybridization (FISH). 
      Single whole chromosome painting (WCP) probes, specific for chromosomes 1-4, 
      6-10, 12, 14 and X were hybridized separately. A human pancentromeric DNA-probe 
      was used simultaneously for unequivocal centromere detection. For both aberration 
      types, symmetrical translocations and dicentrics, a significant deviation from a 
      DNA-proportional distribution was found. In general, chromosomes with a higher 
      DNA content (chromosomes 1-3, 6 and 7) were less frequently involved in the 
      formation of symmetrical translocations and dicentrics than expected according to 
      their DNA-content, whereas smaller chromosomes were more frequently involved. The 
      only exception was chromosome 4, exhibiting the highest translocation frequency 
      of all chromosomes analysed. Ratios of the yields of symmetrical translocations 
      to the yields of dicentrics varied between 0.9 and 1.8 for the single 
      chromosomes. The present results substantiate our previous data obtained with 
      identical chromosomes but examined in four different triple combinations.
FAU - Knehr, S
AU  - Knehr S
AD  - GSF-Forschungszenstrum fur Umwelt und Gesundheit, Institut fur Strahlenbiologie, 
      Neuherberg, Germany.
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Braselmann, H
AU  - Braselmann H
FAU - Nahrstedt, U
AU  - Nahrstedt U
FAU - Bauchinger, M
AU  - Bauchinger M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*radiation effects
MH  - DNA/*analysis/genetics/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/radiation effects/ultrastructure
MH  - Translocation, Genetic/radiation effects
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1080/095530096144851 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1996 Oct;70(4):385-92. doi: 10.1080/095530096144851.