PMID- 8862599
OWN - NLM
STAT- MEDLINE
DCOM- 19970123
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 34
IP  - 9
DP  - 1996 Sep
TI  - In vivo detection of porcine reproductive and respiratory syndrome virus RNA by 
      in situ hybridization at different times postinfection.
PG  - 2280-6
AB  - We studied the distribution of porcine reproductive and respiratory syndrome 
      virus (PRRSV) RNA in tissues by in situ hybridization at different times 
      postinfection (p.i.). The probe used for in situ hybridization was prepared by 
      reverse transcription of PRRSV RNA, followed by PCR amplification of the cDNA. 
      The sequence amplified corresponded to 433 bp from PRRSV open reading frame 7, 
      which is contained in the nucleocapsid protein gene and which is highly conserved 
      in both European and American strains (H. Mardassi, L. Wilson, S. Mounir, and S. 
      Dea, J. Clin. Microbiol. 32:2197-2203, 1994). An immunohistochemical technique 
      was used to detect PRRSV antigen in tissue from virus-infected animals by using a 
      monoclonal antibody specific for the PRRSV nucleocapsid protein (E.A. Nelson, J. 
      Christopher-Hennings, T. Drew, G. Wensvoort, J.E. Collins, and D.A. Benfield, J. 
      Clin. Microbiol. 31:3184-3189, 1993). The detection of PRRSV RNA was conducted in 
      tissues of 6-week-old pigs that had been infected with one of three different 
      field PRRSV isolates and collected at times ranging from 4 to 42 days p.i. 
      Hybridization signals specific for PRRSV RNA were detected in lung, lymphoid 
      tissues, alveolar macrophages (obtained by lavage at the time of necropsy), 
      Peyer's patches, and kidney. The PRRSV-positive cells in these tissues appeared 
      to be predominantly macrophages. In lung tissue we also obtained evidence 
      suggesting the involvement of type II pneumocytes in the replication of PRRSV. 
      During the acute period of infection there was a close correlation between the 
      detection of RNA and the detection of nucleocapsid protein in individual cells. 
      At later times p.i. (28 and 42 days p.i.), instead, more cells containing only 
      PRRSV RNA than those containing PRRSV RNA and also expressing PRRSV nucleocapsid 
      protein were detected. These results suggest that PRRSV RNA might persist in the 
      tissues of infected animals for a longer time than PRRSV antigen expression.
FAU - Sur, J H
AU  - Sur JH
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln 
      68503-0905, USA.
FAU - Cooper, V L
AU  - Cooper VL
FAU - Galeota, J A
AU  - Galeota JA
FAU - Hesse, R A
AU  - Hesse RA
FAU - Doster, A R
AU  - Doster AR
FAU - Osorio, F A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - In Situ Hybridization
MH  - Porcine Reproductive and Respiratory Syndrome/physiopathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - RNA, Viral/*analysis
MH  - Swine
MH  - Time Factors
PMC - PMC229232
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
PMCR- 1996/09/01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PHST- 1996/09/01 00:00 [pmc-release]
AID - 10.1128/jcm.34.9.2280-2286.1996 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1996 Sep;34(9):2280-6. doi: 10.1128/jcm.34.9.2280-2286.1996.