PMID- 8863163
OWN - NLM
STAT- MEDLINE
DCOM- 19961227
LR  - 20190503
IS  - 0022-2593 (Print)
IS  - 1468-6244 (Electronic)
IS  - 0022-2593 (Linking)
VI  - 33
IP  - 8
DP  - 1996 Aug
TI  - Investigation of an interleukin-4 promoter polymorphism for associations with 
      asthma and atopy.
PG  - 689-92
AB  - The cytokine cluster located on chromosome 5 has been shown by linkage studies to 
      play a role in the genetic determination of circulating immunoglobulin E (IgE) 
      levels in atopic subjects. In the study presented here, the reported chromosome 5 
      linkage has been investigated in two sets of subjects. The first consisted of a 
      general population sample of 230 nuclear families (n = 1004) from Busselton, a 
      small West Australian country town. The second group consisted of 124 unrelated 
      atopic asthmatics and 59 unrelated non-atopic, non-asthmatic controls, all 
      resident in the Oxfordshire Regional Health Authority area in the United Kingdom. 
      A previously reported interleukin-4 (II-4) promoter polymorphism (-590 C-->T) was 
      analysed in these populations by a newly designed method of specific PCR 
      amplification and BsmFI restriction endonuclease digestion. In the Busselton 
      population the polymorphism was shown to be weakly associated with specific IgE 
      to house dust mite (Mann-Whitney-U test, p = 0.013) and to wheeze (MWU test, p = 
      0.029), but not with specific IgE to grass pollen, total serum IgE, bronchial 
      hyperresponsiveness, eosinophil count, or asthma. In the Oxfordshire subjects 
      there were no statistically significant associations with any measure of asthma 
      or atopy. These data show that the -590 C-->T II-4 promoter polymorphism is only 
      weakly associated with certain measures of asthma and atopy in some subjects. It 
      was specifically not associated with serum IgE concentration or asthma in either 
      of the two groups in this study.
FAU - Walley, A J
AU  - Walley AJ
AD  - Nuffield Department of Medicine, John Radcliffe Hospital, Oxford, UK.
FAU - Cookson, W O
AU  - Cookson WO
LA  - eng
SI  - GENBANK/M23442
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Med Genet
JT  - Journal of medical genetics
JID - 2985087R
RN  - 0 (Allergens)
RN  - 0 (Dust)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens
MH  - Animals
MH  - Asthma/*genetics/immunology
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Dust
MH  - Female
MH  - Gene Frequency
MH  - Genetic Linkage
MH  - Humans
MH  - Hypersensitivity, Immediate/*genetics/immunology
MH  - Immunoglobulin E/analysis/blood
MH  - Interleukin-4/*genetics
MH  - Male
MH  - Mites
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - *Polymorphism, Restriction Fragment Length
MH  - Promoter Regions, Genetic/*genetics
MH  - Respiratory Sounds/genetics
MH  - United Kingdom
MH  - Western Australia
PMC - PMC1050705
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
PMCR- 1996/08/01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PHST- 1996/08/01 00:00 [pmc-release]
AID - 10.1136/jmg.33.8.689 [doi]
PST - ppublish
SO  - J Med Genet. 1996 Aug;33(8):689-92. doi: 10.1136/jmg.33.8.689.