PMID- 8863511
OWN - NLM
STAT- MEDLINE
DCOM- 19961202
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 67
IP  - 5
DP  - 1996 Nov
TI  - Interleukin-1 beta and tumor necrosis factor-alpha induce expression of alpha 
      1-antichymotrypsin in human astrocytoma cells by activation of nuclear 
      factor-kappa B.
PG  - 2039-44
AB  - The protease inhibitor alpha 1-antichymotrypsin (ACT) has been suggested to be 
      involved in the etiology of Alzheimer's disease (AD). Increased levels of ACT 
      have been found in serum and brains of AD patients, and ACT has been proposed to 
      regulate beta-amyloid fibril formation in vitro. To gain insight into the 
      regulation of ACT in the brain, we investigated the signal transduction pathways 
      involved in ACT gene expression and protein synthesis in the human astrocytoma 
      cell line U373. This cell line has previously been shown to respond with strong 
      ACT synthesis on stimulation with interleukin-1 beta (IL-1 beta) or tumor 
      necrosis factor-alpha (TNF alpha). Here, we describe that both IL-1 beta and TNF 
      alpha activate the transcription factor nuclear factor-kappa B (NF-kappa B) via 
      production of reactive oxygen intermediates resulting in ACT expression. In 
      addition, we show that neither protein kinase C nor protein kinase A is involved 
      in IL-1 beta- or TNF alpha-induced ACT expression. These results suggest that 
      activation of NF-kappa B may be one possible cause of increased ACT levels in AD 
      and provide a basis for the development of drugs used for the modulation of 
      inflammatory processes occurring in AD.
FAU - Lieb, K
AU  - Lieb K
AD  - Abteilung Psychiatrie und Psychotherapie, Universitat Freiburg, Germany.
FAU - Fiebich, B L
AU  - Fiebich BL
FAU - Schaller, H
AU  - Schaller H
FAU - Berger, M
AU  - Berger M
FAU - Bauer, J
AU  - Bauer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Interleukin-1)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (alpha 1-Antichymotrypsin)
RN  - 1F7A44V6OU (Colforsin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Astrocytoma/*metabolism
MH  - Blotting, Northern
MH  - Bucladesine/pharmacology
MH  - Cell Line
MH  - Colforsin/pharmacology
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - NF-kappa B/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Recombinant Proteins/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription, Genetic/*drug effects
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - alpha 1-Antichymotrypsin/*biosynthesis
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1996.67052039.x [doi]
PST - ppublish
SO  - J Neurochem. 1996 Nov;67(5):2039-44. doi: 10.1046/j.1471-4159.1996.67052039.x.