PMID- 8863658
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 149
IP  - 4
DP  - 1996 Oct
TI  - Localization of simian immunodeficiency virus nucleic acid and antigen in brains 
      of fetal macaques inoculated in utero.
PG  - 1097-104
AB  - Neurological dysfunction has been shown to be associated with human 
      immunodeficiency virus (HIV) infection. The incidence of these abnormalities is 
      greater in HIV-infected children when compared with adults, and the patterns of 
      neurological disease are also known to differ from those observed in the adult 
      population. The reasons for these differences are unclear but are most likely 
      related to the immaturity of the host's immune and central nervous systems at the 
      time of infection. This is thought to be particularly true for infants infected 
      with HIV prenatally. To examine these questions, the brains of fetal rhesus 
      macaques that were infected with SIVmac251 at various time points in utero were 
      examined. Direct fetal inoculations were performed on gestational day (GD) 65 (n 
      = 8; early second trimester), GD 110 (n = 4; early third trimester) and GD 130 (n 
      = 2; mid third trimester), with harvest of fetal tissues on GD 80, 100, 130, or 
      145. Eleven sham controls were included with harvest at correlative time points. 
      Specimens were examined by routine histology, immunohistochemistry, and in situ 
      hybridization to localize viral antigens and SIV nucleic acid. Histologically, 
      scattered glial nodules, spongiosis, and mineralization were found in the basal 
      ganglia and deep white matter in 4 of the 14 fetuses (3 inoculated on GD 65 and 
      one on GD 110). These fetuses and those without histological lesions had viral 
      nucleic acid and SIV antigen in the stroma of the choroid plexus, meninges, and 
      external granular layer of the cerebellum and in columns of cells in the cortical 
      plate. In contrast to juvenile and adult macaques, very few SIV-positive 
      perivascular mononuclear cells were present. These findings suggest that SIV has 
      a different distribution in the brain of fetal macaques after direct infection 
      when compared with adult or juvenile animals. Furthermore, the results of these 
      studies suggest that differences in neurological disease between pediatric and 
      adult patients with acquired immune deficiency syndrome are most likely related 
      to the time of infection.
FAU - Lane, J H
AU  - Lane JH
AD  - New England Regional Primate Research Center, Harvard Medical School, 
      Southborough, Massachusetts 01772, USA.
FAU - Tarantal, A F
AU  - Tarantal AF
FAU - Pauley, D
AU  - Pauley D
FAU - Marthas, M
AU  - Marthas M
FAU - Miller, C J
AU  - Miller CJ
FAU - Lackner, A A
AU  - Lackner AA
LA  - eng
GR  - NS30769/NS/NINDS NIH HHS/United States
GR  - RR00168/RR/NCRR NIH HHS/United States
GR  - RR00169/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*isolation & purification
MH  - Brain/embryology/pathology/*virology
MH  - DNA, Viral/*isolation & purification
MH  - Disease Models, Animal
MH  - Macaca mulatta
MH  - RNA, Viral/*isolation & purification
MH  - Simian Acquired Immunodeficiency Syndrome/immunology/pathology/*virology
MH  - Simian Immunodeficiency Virus/genetics/immunology/*isolation & purification
PMC - PMC1865194
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
PMCR- 1997/04/01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
PHST- 1997/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1996 Oct;149(4):1097-104.