PMID- 886402
OWN - NLM
STAT- MEDLINE
DCOM- 19770929
LR  - 20151119
IS  - 0022-3395 (Print)
IS  - 0022-3395 (Linking)
VI  - 63
IP  - 4
DP  - 1977 Aug
TI  - Cytofluorograf detection of Plasmodium yoelii, Trypanosoma gambiense, and 
      Trypanosoma equiperdum by laser excited fluorescence of stained rodent blood.
PG  - 593-8
AB  - Samples of rat blood infected with Plasmodium yoelii (3% parasitized 
      erythrocytes), Trypanosoma gambiense, or Trypanosoma equiperdum (greater than 50 
      trypanosomes per microscope field at 400 X) were fixed with 0.5% glutaraldehyde 
      in phosphate buffered saline, then stained with acridine orange (AO) at 10(-4), 
      10(-5), or 10(-6) M for 0 to 15 min at 5 C or 25 C and/or ethidium bromide (EB) 
      at 0.05 mg/ml for 20 min at 25 C. Stained cells were analyzed with a laser 
      Cytofluorograf (Bio/Physics Systems, Inc.) to determine if parasites could be 
      detected and differentiated from blood cells by their fluorescent 
      characteristics. Samples of uninfected rat blood with and without leukocytes and 
      P. yoelii-infected blood without leukocytes were treated similarly. In addition, 
      suspensions of T. gambiense and T. equiperdum without all blood cells were 
      stained with AO or EB and analyzed with the Cytofluorograf, as were mixed 
      suspensions of both trypanosome species. EB- but not AO-stained P. 
      yoelii-infected erythrocytes had fluorescent characteristics different from most 
      blood cells. Neither AO- nor EB-stained T. gambiense or T. equiperdum could be 
      differentiated from host blood cells or from each other. The results are 
      discussed with respect to the use of laser flow systems in the detection and 
      analysis of bloodstream dwelling protozoan parasites.
FAU - Jackson, P R
AU  - Jackson PR
FAU - Winkler, D G
AU  - Winkler DG
FAU - Kimzey, S L
AU  - Kimzey SL
FAU - Fisher, F M Jr
AU  - Fisher FM Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
RN  - 0 (Acridines)
RN  - 0 (Fluorescent Dyes)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Acridines
MH  - Animals
MH  - Blood/*parasitology
MH  - Ethidium
MH  - Fluorescent Dyes
MH  - Lasers
MH  - Malaria/parasitology
MH  - Male
MH  - Mice
MH  - *Plasmodium
MH  - *Trypanosoma
MH  - *Trypanosoma brucei gambiense
MH  - Trypanosomiasis/parasitology
EDAT- 1977/08/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1977/08/01 00:00
PHST- 1977/08/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1977/08/01 00:00 [entrez]
PST - ppublish
SO  - J Parasitol. 1977 Aug;63(4):593-8.