PMID- 8864132
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 60
IP  - 4
DP  - 1996 Oct
TI  - The inhibitory effect of prostaglandin E2 on rat neutrophil aggregation.
PG  - 480-6
AB  - Rat peritoneal neutrophils stimulated by N-formyl-methionyl-leucyl-phenylalanine 
      (fMLP) produce an aggregation response that can be inhibited by prostaglandin E2 
      (PGE2) with an IC50 value of 2.6 x 10(-9) M. Although PGE2 can stimulate [3H]cAMP 
      production in neutrophils (EC50 4.3 x 10(-8) M), the anti-aggregation response 
      cannot be significantly attenuated by inhibitors of adenylate cyclase or protein 
      kinase A, neither can it be potentiated by inhibition of phosphodiesterase 
      activity. Despite these observations, it still remains possible that 
      PGE2-mediated inhibition of rat neutrophil aggregation is a cAMP-dependent 
      response mediated by highly localized changes in neutrophil cAMP. The inhibitory 
      effect of PGE2 does not appear to depend on effects on intracellular calcium or 
      K(ATP) channels. Similarities exist between PGE2 and the profile of activity of 
      phosphatidylinositol 3-kinase (PI 3-kinase) inhibitors, suggesting that PI 
      3-kinase is a possible target for PGE2 action in rat neutrophils.
FAU - Wise, H
AU  - Wise H
AD  - Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong 
      Kong.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Androstadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoquinolines)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (Potassium Channels)
RN  - 0 (Pyrrolidinones)
RN  - 0 (Sulfonamides)
RN  - 17318-31-9 (9-(tetrahydro-2-furyl)-adenine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - JAC85A2161 (Adenine)
RN  - K676NL63N7 (Rolipram)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - SX6K58TVWC (Glyburide)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Ascitic Fluid/cytology
MH  - Cell Aggregation/*drug effects
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Cyclic AMP/physiology
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
MH  - Dinoprostone/*pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Glyburide/pharmacology
MH  - Isoquinolines/pharmacology
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects
MH  - Phosphatidylinositol 3-Kinases
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors
MH  - Potassium Channels/drug effects
MH  - Pyrrolidinones/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rolipram
MH  - *Sulfonamides
MH  - Wortmannin
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1002/jlb.60.4.480 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 Oct;60(4):480-6. doi: 10.1002/jlb.60.4.480.