PMID- 8866867
OWN - NLM
STAT- MEDLINE
DCOM- 19961213
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 491 ( Pt 2)
IP  - Pt 2
DP  - 1996 Mar 1
TI  - Phospholipase A2 and protein kinase C contribute to myofilament sensitization to 
      5-HT in the rabbit mesenteric artery.
PG  - 447-53
AB  - 1. Calcium (Ca2+, 0.1-100 microM) stimulated concentration-dependent contractions 
      in small strips from the rabbit mesenteric artery in which the smooth muscle 
      cells had been permeabilized with Staphylococcus aureus alpha-toxin. 2. 
      5-Hydroxytryptamine (5-HT) and phenylephrine, each in the presence of 10 microM 
      guanosine 5'-triphosphate (GTP), concentration-dependently stimulated additional 
      contractions in strips sub-maximally contracted by the presence of a buffered 
      concentration of calcium (0.3 microM). All the additional contraction was 
      abolished with the selective inhibitor of protein kinase C, Ro 31-8220 (10 
      microM). 3. Quinacrine (10-50 microM), an inhibitor of phospholipase A2, 
      selectively inhibited the sensitization to 5-HT, but did not alter the 
      sensitization to either phenylephrine or GTP. 4. Myofilament sensitization to 
      calcium was mimicked by exogenous arachidonic acid (300 microM, in the presence 
      of indomethacin, miconazole and BW755c) and the stable analogue of arachidonic 
      acid, 5,8,11,14-eicosatetrayonic acid (ETYA, 100 microM), and in both cases did 
      not require the additional presence of GTP. Ro 31-8220, but not quinacrine, 
      reduced the sensitization to arachidonic acid by around 30%. 5. These results 
      indicate that G protein-linked myofilament sensitization to calcium in the 
      mesenteric artery that follows the activation of 5-HT receptors, but not alpha 
      1-receptors, involves phospholipase A2. The sensitization stimulated by each of 
      these different receptors, and a component of the response to arachidonic acid, 
      also appears to involve the activation of protein kinase C.
FAU - Parsons, S J
AU  - Parsons SJ
AD  - Department of Pharmacology, University of Bristol, UK.
FAU - Sumner, M J
AU  - Sumner MJ
FAU - Garland, C J
AU  - Garland CJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 1WS297W6MV (Phenylephrine)
RN  - 333DO1RDJY (Serotonin)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - H0C805XYDE (Quinacrine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Mesenteric Arteries/*drug effects
MH  - Phenylephrine/pharmacology
MH  - Phospholipases A/*pharmacology
MH  - Phospholipases A2
MH  - Protein Kinase C/*pharmacology
MH  - Quinacrine/pharmacology
MH  - Rabbits
MH  - Serotonin/*pharmacology
PMC - PMC1158738
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
PMCR- 1996/03/01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PHST- 1996/03/01 00:00 [pmc-release]
AID - 10.1113/jphysiol.1996.sp021228 [doi]
PST - ppublish
SO  - J Physiol. 1996 Mar 1;491 ( Pt 2)(Pt 2):447-53. doi: 
      10.1113/jphysiol.1996.sp021228.