PMID- 8867596
OWN - NLM
STAT- MEDLINE
DCOM- 19970103
LR  - 20071114
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 16
IP  - 6
DP  - 1995 Nov-Dec
TI  - Effect of TGF-beta 1, TGF-alpha, and EGF on cell proliferation and cell death in 
      rat ventral prostatic epithelial cells in culture.
PG  - 482-90
AB  - A tissue culture system for rat prostatic epithelial cells was developed, and the 
      effect of epidermal growth factor (EGF), transforming growth factor alpha 
      (TGF-alpha), and transforming growth factor beta 1 (TGF-beta 1) on these cells 
      was evaluated. The primary culture was prepared by DNAse/collagenase dissociation 
      of minced ventral prostates. Cells were initially plated in RPMI-1640 medium 
      containing 10% fetal bovine serum to allow the preferential attachment of stromal 
      cells. Twenty-four hours later, the unattached epithelial cells were replated in 
      WAJC-404 medium supplemented with insulin (5 micrograms/ml), transferrin (5 
      micrograms/ml), and selenious acid (5 ng/ml). Bovine pituitary extract (BPE) (30 
      micrograms/ml), EGF (10 ng/ml), and TGF-beta 1 (0, 0.1, and 1.0 ng/ml) were added 
      either alone or in combination according to experimental requirements. The rate 
      of cell proliferation was assessed by counting the total cell number and by 
      [3H]thymidine incorporation. Prostatic epithelial cells exhibited a bell-shaped 
      growth curve in a span of 7-8 days, with a growth peak at day 3 or 4 of culture. 
      Treatment of cells with EGF or TGF-alpha resulted in a concentration-dependent 
      increase in cell growth, whereas addition of TGF-beta 1 into the culture resulted 
      in an inhibition of cell proliferation that could be reversed with increasing 
      concentrations of EGF. Cell death was assessed using the terminal 
      deoxynucleotidyl transferase (TdT)-mediated immunoperoxidase-digoxigenin nick end 
      labeling technique and the trypan blue exclusion test. Epithelial cells cultured 
      in media containing EGF had the lowest incidence of cell death. Cells cultured in 
      the absence of EGF demonstrated a marked increase in cells undergoing cell death. 
      The addition of TGF-beta 1 into the EGF-depleted medium caused a further increase 
      of cell death. These results indicated that cell proliferation and cell death in 
      rat prostatic epithelial cells in culture could be modulated by EGF and TGF-beta 
      1. The former stimulated cell proliferation and prevented cell death, whereas the 
      latter inhibited proliferation in the presence or absence of EGF and induced cell 
      death.
FAU - Ilio, K Y
AU  - Ilio KY
AD  - Department of Urology, Northwestern University Medical School, Chicago, Illinois 
      60611, USA.
FAU - Sensibar, J A
AU  - Sensibar JA
FAU - Lee, C
AU  - Lee C
LA  - eng
GR  - DK39250/DK/NIDDK NIH HHS/United States
GR  - DK43541/DK/NIDDK NIH HHS/United States
GR  - DK47561/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Transforming Growth Factor beta)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/*pharmacology
MH  - Epithelial Cells
MH  - Epithelium/drug effects
MH  - Male
MH  - Prostate/cytology/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor alpha/*pharmacology
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - J Androl. 1995 Nov-Dec;16(6):482-90.