PMID- 8867598
OWN - NLM
STAT- MEDLINE
DCOM- 19970103
LR  - 20181130
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 16
IP  - 6
DP  - 1995 Nov-Dec
TI  - Testosterone and spermatogenesis: evidence that androgens regulate cellular 
      secretory mechanisms in stage VI-VIII seminiferous tubules from adult rats.
PG  - 499-509
AB  - The aim of this study was to investigate the effect of testosterone manipulation 
      on the quantitative synthesis and secretion of a number of specific proteins 
      produced by seminiferous tubules (ST) isolated at stages VI-VIII of the 
      spermatogenic cycle from adult rats. The proteins selected were derived from 
      different cellular sources. ST were isolated from control rats, from rats treated 
      4 days earlier with ethane dimethane sulfonate (EDS) to induce complete 
      testosterone withdrawal by the destruction of the Leydig cells, and from 
      EDS-treated rats injected with testosterone esters (TE) in order to maintain 
      quantitatively normal spermatogenesis. Two-dimensional sodium dodecyl sulfate 
      polyacrylamide gel electrophoresis, combined with computerized image analysis, 
      was used to analyze 35S-methionine-labeled intracellular and secreted proteins. 
      Testosterone withdrawal did not affect to any significant degree the total 
      synthesis of any of the proteins studied. Similarly, the secretion of the major 
      known Sertoli cell proteins SGP-1 and SGP-2, together with a third putative 
      Sertoli cell protein, all of which appeared to be secreted constitutively, was 
      also not affected to any major degree by EDS treatment. In contrast, the 
      secretion of another probable Sertoli cell protein, together with six proteins 
      found to be secreted by germ cells and one protein that appeared to derive from 
      more than one cellular source, was reduced dramatically by testosterone 
      withdrawal, but was maintained by treatment with EDS+TE. All of the affected 
      proteins appeared to be secreted in a regulated manner. Our results confirm that 
      testosterone manipulation has little or no effect on either total protein 
      synthesis by ST, or on the secretion of the major Sertoli cell secretory 
      proteins, at stages VI-VIII of the spermatogenic cycle, but suggest strongly that 
      testosterone regulation of ST protein secretion at these stages is mediated by an 
      effect on the regulated secretory pathways. Our findings also demonstrate that 
      the secretion, not only of Sertoli cell proteins, but also of those secreted by 
      germ cells, is androgen-regulated.
FAU - McKinnell, C
AU  - McKinnell C
AD  - MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh 
      Scotland, United Kingdom.
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Actins)
RN  - 0 (Androgens)
RN  - 0 (Proteins)
RN  - 3XMK78S47O (Testosterone)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Actins/chemistry/metabolism
MH  - Amino Acid Sequence
MH  - Androgens/*physiology
MH  - Animals
MH  - Autoradiography
MH  - Body Fluids/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Germ Cells/metabolism
MH  - L-Lactate Dehydrogenase/chemistry/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Rete Testis/metabolism
MH  - Seminiferous Tubules/cytology/*metabolism
MH  - Sertoli Cells/metabolism
MH  - Spermatogenesis/*physiology
MH  - Testosterone/*physiology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - J Androl. 1995 Nov-Dec;16(6):499-509.