PMID- 8869915
OWN - NLM
STAT- MEDLINE
DCOM- 19970115
LR  - 20190830
IS  - 0047-6374 (Print)
IS  - 0047-6374 (Linking)
VI  - 90
IP  - 2
DP  - 1996 Sep 18
TI  - Age-related aneuploidy analysis of human blood cells in vivo by fluorescence in 
      situ hybridization (FISH).
PG  - 145-56
AB  - All prior studies on human age-related chromosomal analysis were done using only 
      metaphase figures derived from lymphocyte cultures in vitro. However, we believe 
      that this procedure may provide only partial information, since the chromosomal 
      abnormalities probably hidden in non-dividing and/or terminally differentiated 
      leukocytes will not be detected by this method. We, therefore, have attempted to 
      analyze the nature and extent of chromosome-specific aneuploidy at interphase by 
      fluorescence in situ hybridization (FISH) in differentiated myeloid cells in vivo 
      derived from various age-group people. Our data from an analysis of 30,032 cells 
      derived from 12 healthy donors indicate that there is an increase in the mean 
      percentages of myeloid cells with chromosome-specific aneuploidy in the older 
      groups as opposed to that of the younger groups. This increase is applicable to 
      all the three cell types examined (promyelocytes, metamyelocytes and polymorphs). 
      In both the younger and older females, the relatively higher mean frequencies of 
      cells with aneuploidy were noted for chromosome nos. 4, 6 and the X, whereas the 
      lowest mean frequencies of cells with aneuploidy were consistently observed for 
      chromosome no. 3. In the younger and older male donors, similar to the female 
      donors except the X chromosome the higher percentages of aneuploid cells were 
      observed for chromosome nos. 4 and 6 whereas the lowest mean frequencies of 
      aneuploid cells were noted for chromosome no. 3. Among the five autosomes 
      studied, chromosome no. 3 consistently yielded the lowest frequency of aneuploidy 
      in all the three cell types derived from both the younger and older groups of 
      males and females. This could presumably mean that the maintenance of a very high 
      frequency of cells with disomy for chromosome no. 3 might be more beneficial for 
      the process of survival and/or differentiation of myeloid cells as compared to 
      that of other autosomes such as chromosome nos. 4 and 6. Among the five autosomes 
      studied, chromosome no. I exhibited the highest rate of increment in the 
      aneuploidy values of both males and females with advancing age.
FAU - Mukherjee, A B
AU  - Mukherjee AB
AD  - Department of Biological Sciences, Fordham University, Bronx, NY 10458, USA.
FAU - Alejandro, J
AU  - Alejandro J
FAU - Payne, S
AU  - Payne S
FAU - Thomas, S
AU  - Thomas S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mech Ageing Dev
JT  - Mechanisms of ageing and development
JID - 0347227
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aging/blood/*genetics
MH  - *Aneuploidy
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - DNA Probes
MH  - DNA, Satellite/genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukocytes/*physiology
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
EDAT- 1996/09/18 00:00
MHDA- 1996/09/18 00:01
CRDT- 1996/09/18 00:00
PHST- 1996/09/18 00:00 [pubmed]
PHST- 1996/09/18 00:01 [medline]
PHST- 1996/09/18 00:00 [entrez]
AID - 0047-6374(96)01762-9 [pii]
AID - 10.1016/0047-6374(96)01762-9 [doi]
PST - ppublish
SO  - Mech Ageing Dev. 1996 Sep 18;90(2):145-56. doi: 10.1016/0047-6374(96)01762-9.