PMID- 88720
OWN - NLM
STAT- MEDLINE
DCOM- 19790917
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 6
IP  - 7
DP  - 1979 Jun 11
TI  - Cloning of an almost full-length chicken conalbumin double-stranded cDNA.
PG  - 2435-52
AB  - Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a 
      laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The 
      dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 
      which had been repaired with DNA polymerase I to create Taq I sites on each side 
      of the inserted fragment. After bacterial transformation, one hybrid recombinant, 
      pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown 
      to hybridize specifically to the RNA which is translated into conalbumin. 
      Electron microscopic examination of hybrid molecules between con-mRNA and 
      pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length 
      double-stranded transcript of conalbumin mRNA.
FAU - Cochet, M
AU  - Cochet M
FAU - Perrin, F
AU  - Perrin F
FAU - Gannon, F
AU  - Gannon F
FAU - Krust, A
AU  - Krust A
FAU - Chambon, P
AU  - Chambon P
FAU - McKnight, G S
AU  - McKnight GS
FAU - Lee, D C
AU  - Lee DC
FAU - Mayo, K E
AU  - Mayo KE
FAU - Palmiter, R
AU  - Palmiter R
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Recombinant)
RN  - 0 (RNA, Messenger)
RN  - 1391-06-6 (Conalbumin)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chickens
MH  - Conalbumin/biosynthesis
MH  - DNA Restriction Enzymes
MH  - DNA, Recombinant/*biosynthesis
MH  - Microscopy, Electron
MH  - Nucleic Acid Conformation
MH  - Nucleic Acid Hybridization
MH  - Oviducts/*metabolism
MH  - Plasmids
MH  - Protein Biosynthesis
MH  - RNA, Messenger/*metabolism
MH  - RNA-Directed DNA Polymerase
PMC - PMC327864
EDAT- 1979/06/11 00:00
MHDA- 1979/06/11 00:01
CRDT- 1979/06/11 00:00
PHST- 1979/06/11 00:00 [pubmed]
PHST- 1979/06/11 00:01 [medline]
PHST- 1979/06/11 00:00 [entrez]
AID - 10.1093/nar/6.7.2435 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1979 Jun 11;6(7):2435-52. doi: 10.1093/nar/6.7.2435.