PMID- 8872602
OWN - NLM
STAT- MEDLINE
DCOM- 19970116
LR  - 20191024
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 62
IP  - 3
DP  - 1996 Sep 1
TI  - Monocyte chemoattractant protein-1 gene expression in injured pig artery 
      coincides with early appearance of infiltrating monocyte/macrophages.
PG  - 303-13
AB  - Monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) are potent 
      chemokines which attract circulating monocytes and neutrophils respectively to 
      inflamed tissues. JE/MCP-1 gene expression has been previously studied in rabbit 
      aortae after endothelial denudation and the rapid appearance of this transcript 
      was thought to precede emigration of phagocytes. We now report MCP-1 gene 
      expression following de-endothelialization of iliac arteries in the pig, a 
      species which can develop spontaneous atherosclerosis. Using Northern blot 
      analysis, we demonstrated that MCP-1 mRNA was rapidly induced in pig arteries at 
      2 h and continued to increase to reach a maximum at 8 h before returning to low 
      levels at 16-24 h after injury. The increase seen for MCP-1 mRNA at 8 h was also 
      observed for IL-8 mRNA but was not apparent for growth-related gene expressions, 
      urokinase-type plasminogen activator (u-PA), and plasminogen activator 
      inhibitor-1 (PAI-1). Since smooth muscle cells, endothelial cells, and phagocytes 
      are all capable of expressing MCP-1, we examined pig arteries for immunostaining 
      using a monoclonal antibody to human MCP-1 (5D3-F7). At 8 h after injury, the 
      predominant cell type staining positive for MCP-1 was the monocyte/macrophage. 
      Staining was also observed in occasional scattered neutrophils, but MCP-1 protein 
      could not be detected in smooth muscle cells or on extracellular matrix within 
      the sensitivity constraints posed by our methodology. Our results are consistent 
      with invading monocyte/macrophages having a major input into the production of 
      this chemokine in the arterial wall following injury. The fact that MCP-1 
      expression accompanied monocyte/macrophage presence in damaged artery, rather 
      than preceding it, is suggestive that continued MCP-1 expression is required for 
      functions other than chemoattraction.
FAU - Wysocki, S J
AU  - Wysocki SJ
AD  - University of Western Australia, Department of Surgery, Fremantle Hospital, 
      Australia.
FAU - Zheng, M H
AU  - Zheng MH
FAU - Smith, A
AU  - Smith A
FAU - Lamawansa, M D
AU  - Lamawansa MD
FAU - Iacopetta, B J
AU  - Iacopetta BJ
FAU - Robertson, T A
AU  - Robertson TA
FAU - Papadimitriou, J M
AU  - Papadimitriou JM
FAU - House, A K
AU  - House AK
FAU - Norman, P E
AU  - Norman PE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Arteries/*injuries/*metabolism
MH  - Catheterization/adverse effects
MH  - Chemokine CCL2/biosynthesis/*genetics/immunology
MH  - Endothelium, Vascular/injuries/physiology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Hyperplasia
MH  - Iliac Artery/injuries/pathology/physiopathology
MH  - Interleukin-8/genetics
MH  - Macrophages/*physiology
MH  - Male
MH  - Monocytes/*physiology
MH  - Plasminogen Activator Inhibitor 1/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Swine
MH  - Time Factors
MH  - Urokinase-Type Plasminogen Activator/genetics
EDAT- 1996/09/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-4644(199609)62:3<303::AID-JCB1>3.0.CO;2-V [pii]
AID - 10.1002/(sici)1097-4644(199609)62:3<303::aid-jcb1>3.0.co;2-v [doi]
PST - ppublish
SO  - J Cell Biochem. 1996 Sep 1;62(3):303-13. doi: 
      10.1002/(sici)1097-4644(199609)62:3<303::aid-jcb1>3.0.co;2-v.