PMID- 8872647
OWN - NLM
STAT- MEDLINE
DCOM- 19970123
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 81
IP  - 2
DP  - 1996 Aug
TI  - Transcription of the rat skeletal muscle hexokinase II gene is increased by acute 
      exercise.
PG  - 789-93
AB  - A single bout of acute exercise increases hexokinase (HK) II mRNA and enzyme 
      activity [R. M. O'Doherty, D. P. Bracy, H. Osawa, D. H. Wasserman, and D. K. 
      Granner. Am. J. Physiol. 266 (Endocrinol. Metab. 29): E171-E178, 1994]. The 
      present study addresses the mechanism of the increase in HK II mRNA. Male rats 
      undertook a single bout of treadmill exercise and were then killed immediately or 
      after a predetermined recovery period. The gastrocnemius/plantaris muscle 
      complex, composed of mixed fiber types, was excised; the nuclei were isolated; 
      and HK I, HK II, beta-actin, and alpha-tubulin gene transcription rates were 
      measured. Genomic DNA and plasmid DNA were used as positive and negative 
      controls, respectively. Immediately after the cessation of 30, 45, or 90 min of 
      exercise, HK II gene transcription rates were 1.3 +/- 0.3-,2.9 +/- 0.3-, and 4.0 
      +/- 0.6-fold, respectively, above those of sedentary controls. The increases 
      after 45 and 90 min of exercise were statistically significant (P < 0.01). One 
      hour after the cessation of 30 min of exercise, HK II gene transcription was 
      significantly increased (1.40 +/- 0.03-fold; P < 0.05). At all time points, 
      transcription of the HK I, beta-actin, and alpha-tubulin genes was unchanged. We 
      conclude that the exercise-induced increase in HK II gene transcription appears 
      to play a major role in the increase of HK II mRNA and activity.
FAU - O'Doherty, R M
AU  - O'Doherty RM
AD  - Department of Molecular Physiology and Biophysics, Vanderbilt University School 
      of Medicine, Nashville, Tennesse 37232, USA.
FAU - Bracy, D P
AU  - Bracy DP
FAU - Granner, D K
AU  - Granner DK
FAU - Wasserman, D H
AU  - Wasserman DH
LA  - eng
GR  - DK-50277/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Actins)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tubulin)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Actins/biosynthesis/genetics
MH  - Animals
MH  - DNA/biosynthesis
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Glucose/metabolism
MH  - Hexokinase/*biosynthesis/*genetics
MH  - Isoenzymes/*biosynthesis/*genetics
MH  - Male
MH  - Muscle, Skeletal/*enzymology
MH  - Physical Exertion/*physiology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcription, Genetic/*physiology
MH  - Tubulin/biosynthesis/genetics
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1152/jappl.1996.81.2.789 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1996 Aug;81(2):789-93. doi: 10.1152/jappl.1996.81.2.789.