PMID- 88736
OWN - NLM
STAT- MEDLINE
DCOM- 19790925
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 76
IP  - 6
DP  - 1979 Jun
TI  - Virolysis of mouse mammary tumor virus by sera from breast cancer patients.
PG  - 2984-7
AB  - All type C retroviruses are lysed by human serum in apparently 
      antibody-independent, complement-mediated reactions. In contrast, we have now 
      determined that the mouse mammary tumor virus (MMTV), a type B retrovirus, is not 
      disrupted by normal human serum. MMTV was lysed, however, when rabbit antibody to 
      whole MMTV was added to the serum. By taking advantage of this dependence of MMTV 
      lysis on specific antibody, a virolytic assay was developed, based on the 
      measurement of reverse transcriptase released from disrupted virions, to search 
      for evidence of antibodies to MMTV in human sera. Significantly greater virolytic 
      activity was detected in the sera of patients with breast cancer than in sera of 
      patients with benign disease (P less than 0.001) or colorectal cancer (P less 
      than 0.001) or in sera from apparently healthy individuals (P less than 0.002). 
      This assay thus appears to be able to detect a unique attribute, possibly the 
      presence of an antibody crossreacting with MMTV, in serum in patient with breast 
      cancer.
FAU - Witkin, S S
AU  - Witkin SS
FAU - Egeli, R A
AU  - Egeli RA
FAU - Sarkar, N H
AU  - Sarkar NH
FAU - Good, R A
AU  - Good RA
FAU - Day, N K
AU  - Day NK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies, Viral)
RN  - 9007-36-7 (Complement System Proteins)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Antibodies, Viral
MH  - Blood
MH  - Breast Neoplasms/*microbiology
MH  - Complement System Proteins
MH  - Female
MH  - Humans
MH  - Lysogeny
MH  - *Mammary Tumor Virus, Mouse/enzymology
MH  - RNA-Directed DNA Polymerase/metabolism
PMC - PMC383735
EDAT- 1979/06/01 00:00
MHDA- 1979/06/01 00:01
PMCR- 1979/12/01
CRDT- 1979/06/01 00:00
PHST- 1979/06/01 00:00 [pubmed]
PHST- 1979/06/01 00:01 [medline]
PHST- 1979/06/01 00:00 [entrez]
PHST- 1979/12/01 00:00 [pmc-release]
AID - 10.1073/pnas.76.6.2984 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1979 Jun;76(6):2984-7. doi: 10.1073/pnas.76.6.2984.