PMID- 8875432
OWN - NLM
STAT- MEDLINE
DCOM- 19970401
LR  - 20231213
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 7
IP  - 6
DP  - 1996 Jun
TI  - Altered connexin expression after peripheral nerve injury.
PG  - 501-18
AB  - The identification of connexin32 (Cx32) in myelinating Schwann cells and the 
      association of Cx32 mutations with peripheral neuropathies suggest a functional 
      role for gap junction proteins in the nerve. However, after nerve crush injury, 
      Cx32 expression dramatically decreases in Schwann cells in the degenerating 
      region, returning to control levels at newly formed nodes of Ranvier and 
      Schmidt-Lantermann incisures by 30 days. The present study examined increases in 
      expression of other connexins that occur after peripheral nerve injury. A 
      56/58-kDa connexin46 (Cx46) protein species was detected in adult rat sciatic 
      nerve, along with very low levels of Cx46 mRNA. However, by 3 days after crush 
      injury, coincident with changes in Schwann cell phenotype, Cx46 mRNA rapidly 
      increased in the degenerating regions. Additionally, the 56/58-kDa Cx46 protein 
      species present in adult nerve decreased and a 53-kDa Cx46 species, which was 
      also present in cultured Schwann cells, became apparent. Connexin43 (Cx43) mRNA 
      and protein, which was localized to perineurial cells in adult nerve, 
      dramatically increased in endoneurial fibroblasts in the crush and distal regions 
      by 3 days, coincident with macrophage infiltration. By 12 days after injury, Cx43 
      decreased and was comparable to normal nerve. These results suggest that enhanced 
      expression of Cx46 and Cx43, by nonneuronal cells, may be important for the 
      injury and regenerative responses of peripheral nerves.
FAU - Chandross, K J
AU  - Chandross KJ
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 
      10461, USA.
FAU - Kessler, J A
AU  - Kessler JA
FAU - Cohen, R I
AU  - Cohen RI
FAU - Simburger, E
AU  - Simburger E
FAU - Spray, D C
AU  - Spray DC
FAU - Bieri, P
AU  - Bieri P
FAU - Dermietzel, R
AU  - Dermietzel R
LA  - eng
GR  - NS20013/NS/NINDS NIH HHS/United States
GR  - NS20778/NS/NINDS NIH HHS/United States
GR  - NS22956/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (GJA3 protein, human)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Connexin 43/*biosynthesis/genetics
MH  - Connexins/*biosynthesis/genetics
MH  - Fibroblasts/metabolism
MH  - *Gene Expression Regulation
MH  - In Situ Hybridization
MH  - Macrophages/metabolism
MH  - Nerve Crush
MH  - *Nerve Degeneration
MH  - *Nerve Regeneration
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - Neural Conduction
MH  - *Peripheral Nerve Injuries
MH  - Peripheral Nerves/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/metabolism
MH  - Sciatic Nerve/injuries/*physiology
MH  - Gap Junction beta-1 Protein
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - S1044-7431(96)90036-6 [pii]
AID - 10.1006/mcne.1996.0036 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 1996 Jun;7(6):501-18. doi: 10.1006/mcne.1996.0036.