PMID- 8876171 OWN - NLM STAT- MEDLINE DCOM- 19961204 LR - 20191210 IS - 0027-8424 (Print) IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 93 IP - 21 DP - 1996 Oct 15 TI - p300 is a component of an estrogen receptor coactivator complex. PG - 11540-5 AB - The estrogen receptor (ER) is a ligand-dependent transcription factor that regulates expression of target genes in response to estrogen in concert with other cellular signaling pathways. This suggests that the mechanism by which ER transmits an activating signal to the general transcription machinery may include factors that integrate these diverse signals. We have previously characterized the estrogen receptor-associated protein, ERAP160, as a factor that complexes with ER in an agonist-dependent manner. We have now found that the transcriptional coactivator p300 associates with agonist bound ER and augments ligand-dependent activation by ER. Our studies show that an ER coactivator complex involves a direct hormone-dependent interaction between ER and ERAP160, resulting in the recruitment of p300. In addition, antibodies directed against the cloned steroid receptor coactivator 1 (SRC1) recognize ERAP160. The known role of p300 in multiple signal transduction pathways, including those involving the second messenger cAMP, suggests p300 functions as a point of integration between ER and these other pathways. FAU - Hanstein, B AU - Hanstein B AD - Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Boston, MA 02115, USA. FAU - Eckner, R AU - Eckner R FAU - DiRenzo, J AU - DiRenzo J FAU - Halachmi, S AU - Halachmi S FAU - Liu, H AU - Liu H FAU - Searcy, B AU - Searcy B FAU - Kurokawa, R AU - Kurokawa R FAU - Brown, M AU - Brown M LA - eng GR - CA57374/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Intracellular Signaling Peptides and Proteins) RN - 0 (Ligands) RN - 0 (NCOA7 protein, human) RN - 0 (Nuclear Proteins) RN - 0 (Nuclear Receptor Coactivators) RN - 0 (Proteins) RN - 0 (Receptors, Estrogen) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Trans-Activators) RN - 0 (Transcription Factors) RN - EC 2.3.1.48 (Histone Acetyltransferases) RN - EC 2.3.1.48 (NCOA1 protein, human) RN - EC 2.3.1.48 (Nuclear Receptor Coactivator 1) RN - EC 2.5.1.18 (Glutathione Transferase) SB - IM MH - Animals MH - Blotting, Western MH - Breast Neoplasms MH - Cell Line MH - Chlorocebus aethiops MH - Electrophoresis, Polyacrylamide Gel MH - Female MH - Glutathione Transferase/biosynthesis MH - Histone Acetyltransferases MH - Humans MH - Intracellular Signaling Peptides and Proteins MH - Ligands MH - Nuclear Proteins/biosynthesis/isolation & purification/*metabolism MH - Nuclear Receptor Coactivator 1 MH - Nuclear Receptor Coactivators MH - Protein Biosynthesis MH - Proteins/isolation & purification/*metabolism MH - Receptors, Estrogen/*metabolism MH - Recombinant Fusion Proteins/biosynthesis/isolation & purification/metabolism MH - Signal Transduction MH - *Trans-Activators MH - Transcription Factors/biosynthesis/isolation & purification/*metabolism MH - Transcription, Genetic MH - Transfection MH - Tumor Cells, Cultured PMC - PMC38093 EDAT- 1996/10/15 00:00 MHDA- 1996/10/15 00:01 PMCR- 1997/04/15 CRDT- 1996/10/15 00:00 PHST- 1996/10/15 00:00 [pubmed] PHST- 1996/10/15 00:01 [medline] PHST- 1996/10/15 00:00 [entrez] PHST- 1997/04/15 00:00 [pmc-release] AID - 10.1073/pnas.93.21.11540 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11540-5. doi: 10.1073/pnas.93.21.11540.