PMID- 8876609
OWN - NLM
STAT- MEDLINE
DCOM- 19961120
LR  - 20180216
IS  - 0001-5792 (Print)
IS  - 0001-5792 (Linking)
VI  - 96
IP  - 3
DP  - 1996
TI  - Comparative study of three methods to detect free plasma antiplatelet antibodies.
PG  - 135-9
AB  - We have compared three techniques for the detection of plasma circulating 
      antiplatelet antibodies, i.e., the platelet suspension immunofluorescence test 
      (PSIFT), the platelet radioactive antiglobulin test (PRAT), and the monoclonal 
      antibody immobilization of platelet antigens (MAIPA). Frozen plasma samples from 
      patients with idiopathic thrombocytopenic purpura or HIV-associated 
      thrombocytopenia were used in the study. The PSIFT and PRAT showed the 
      appropriate ease of performance necessary for screening purposes. The PSIFT is 
      free of radioactivity hazards, but seemed to be less sensitive than the PRAT. The 
      MAIPA is a useful tool to detect antibodies against glycoproteins (GPs) Ib/IX and 
      IIb/IIIa. However, in comparison to PSIFT and PRAT, MAIPA is more time consuming, 
      requires considerable technical expertise, and the identification of antiplatelet 
      activity is highly dependent on the selection of an appropriate primary anti-GP 
      monoclonal antibody. This could explain the lower prevalence of antiplatelet 
      activity detected by MAIPA, in comparison to the frequency provided by the PSIFT 
      and PRAT.
FAU - Gonzalez-Conejero, R
AU  - Gonzalez-Conejero R
AD  - Unit of Hematology and Hemotherapy, Hospital General Universitario, Murcia, 
      Spain.
FAU - Rivera, J
AU  - Rivera J
FAU - Rosillo, M C
AU  - Rosillo MC
FAU - Lozano, M L
AU  - Lozano ML
FAU - Garcia, V V
AU  - Garcia VV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Acta Haematol
JT  - Acta haematologica
JID - 0141053
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Autoantibodies)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Surface/immunology
MH  - Autoantibodies/*blood
MH  - Blood Platelets/*immunology
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect/methods
MH  - Humans
MH  - *Immunologic Techniques
MH  - Thrombocytopenia/*diagnosis/immunology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000203744 [doi]
PST - ppublish
SO  - Acta Haematol. 1996;96(3):135-9. doi: 10.1159/000203744.