PMID- 8876638
OWN - NLM
STAT- MEDLINE
DCOM- 19961120
LR  - 20041117
IS  - 0278-0232 (Print)
IS  - 0278-0232 (Linking)
VI  - 14
IP  - 2
DP  - 1996 Jun
TI  - Detection of human T lymphotrophic virus type I (HTLV-I) DNA and mRNA in 
      individual cells by polymerase chain reaction (PCR) in situ hybridization (ISH) 
      and reverse transcription (RT)-PCR ISH.
PG  - 91-100
AB  - Human T lymphotrophic virus type I (HTLV-I) proviral DNA and mRNA in the blood 
      obtained directly from HTLV-I infected adult T cell leukemia (ATL) patients were 
      amplified by the polymerase chain reaction (PCR), and reverse transcription 
      (RT)-PCR, and then were hybridized to fluorescein-labelled probes by means of in 
      situ hybridization (ISH). Before the cytospin samples were prepared, heterogenous 
      cell populations were reproducibly resolved into HTLV-I-positive and -negative 
      distributions. Immunohistochemical staining was performed, using anti-fluorescein 
      monoclonal antibody. Microscopic observations demonstrated a preserved cellular 
      morphology. The intranuclear localization of amplified DNA products of proviral 
      HTLV-I by PCR/ISH, and intracytoplasmic localization of amplified DNA of HTLV-I 
      tax/rex mRNA by RT-PCR/ISH were maintained. In this study, about one in 10 HTLV-I 
      provirus integrated cells expressed low copies of tax/rex mRNA. In 
      HTLV-I-negative cell lines, amplified DNA was not observed by either PCR/ISH or 
      RT-PCR/ISH. With the use of this technique it is thus possible to detect 
      single-copy DNA and a few copies of mRNA, and it is therefore possible to study, 
      not only suspended materials, but also other tissue materials for further 
      characterization, in association with the localization of the HTLV-I infected 
      cells.
FAU - Ohshima, K
AU  - Ohshima K
AD  - Department of Internal Medicine, Kagoshima University, Japan.
FAU - Hashimoto, K
AU  - Hashimoto K
FAU - Izumo, S
AU  - Izumo S
FAU - Suzumiya, J
AU  - Suzumiya J
FAU - Kikuchi, M
AU  - Kikuchi M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hematol Oncol
JT  - Hematological oncology
JID - 8307268
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - DNA, Viral/*analysis
MH  - HL-60 Cells
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Leukemia, T-Cell/*microbiology
MH  - Polymerase Chain Reaction/*methods
MH  - Proviruses/genetics
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/*analysis
EDAT- 1996/06/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1002/(SICI)1099-1069(199606)14:2<91::AID-HON574>3.0.CO;2-G [pii]
AID - 10.1002/(SICI)1099-1069(199606)14:2<91::AID-HON574>3.0.CO;2-G [doi]
PST - ppublish
SO  - Hematol Oncol. 1996 Jun;14(2):91-100. doi: 
      10.1002/(SICI)1099-1069(199606)14:2<91::AID-HON574>3.0.CO;2-G.