PMID- 8879214
OWN - NLM
STAT- MEDLINE
DCOM- 19961213
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 184
IP  - 4
DP  - 1996 Oct 1
TI  - Inducible nitric oxide synthase in tangle-bearing neurons of patients with 
      Alzheimer's disease.
PG  - 1425-33
AB  - In Alzheimer's disease (AD), affected neurons accumulate beta amyloid protein, 
      components of which can induce mouse microglia to express the high-output isoform 
      of nitric oxide synthase (NOS2) in vitro. Products of NOS2 can be neurotoxic. In 
      mice, NOS2 is normally suppressed by transforming growth factor beta 1 (TGF-beta 
      1). Expression of TGF-beta 1 is decreased in brains from AD patients, a situation 
      that might be permissive for accumulation of NOS2. Accordingly, we investigated 
      the expression of NOS2 in patients with AD, using three monospecific antibodies: 
      a previously described polyclonal and two new monoclonal antibodies. 
      Neurofibrillary tangle-bearing neurons and neuropil threads contained NOS2 in 
      brains from each of 11 AD patients ranging in age from 47 to 81 years. NOS2 was 
      undetectable in brains from 6 control subjects aged 23-72 years, but was 
      expressed in small amounts in 3 control subjects aged 77-87 years. Thus, human 
      neurons can express NOS2 in vivo. The high-output pathway of NO production may 
      contribute to pathogenesis in AD.
FAU - Vodovotz, Y
AU  - Vodovotz Y
AD  - Laboratory of Chemoprevention, National Institutes of Health, Bethesda, Maryland 
      20892, USA.
FAU - Lucia, M S
AU  - Lucia MS
FAU - Flanders, K C
AU  - Flanders KC
FAU - Chesler, L
AU  - Chesler L
FAU - Xie, Q W
AU  - Xie QW
FAU - Smith, T W
AU  - Smith TW
FAU - Weidner, J
AU  - Weidner J
FAU - Mumford, R
AU  - Mumford R
FAU - Webber, R
AU  - Webber R
FAU - Nathan, C
AU  - Nathan C
FAU - Roberts, A B
AU  - Roberts AB
FAU - Lippa, C F
AU  - Lippa CF
FAU - Sporn, M B
AU  - Sporn MB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Isoenzymes)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/*enzymology/pathology
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Brain/*enzymology/pathology
MH  - Enzyme Induction
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Isoenzymes/*isolation & purification
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Neurons/*enzymology/pathology
MH  - Nitric Oxide Synthase/*isolation & purification
PMC - PMC2192831
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
PMCR- 1997/04/01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
PHST- 1997/04/01 00:00 [pmc-release]
AID - 97033517 [pii]
AID - 10.1084/jem.184.4.1425 [doi]
PST - ppublish
SO  - J Exp Med. 1996 Oct 1;184(4):1425-33. doi: 10.1084/jem.184.4.1425.