PMID- 8879250
OWN - NLM
STAT- MEDLINE
DCOM- 19961210
LR  - 20231213
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 252
IP  - 4
DP  - 1996 Sep 25
TI  - Characterization and application of soybean YACs to molecular cytogenetics.
PG  - 483-8
AB  - Yeast artificial chromosomes (YACs) are widely used in the physical analysis of 
      complex genomes. In addition to their value in chromosome walking for map-based 
      cloning, YACs represent excellent probes for chromosome mapping using 
      fluorescence in situ hybridization (FISH). We have screened such a library for 
      low-copy-number clones by hybridization to total genomic DNA. Four clones were 
      chosen for chromosome tagging based upon their low or moderate signal. By using 
      degenerate oligonucleotide-primed PCR (DOP-PCR), we were able to use relatively 
      small amounts of soybean YAC DNA, isolated directly by preparative pulsed-field 
      gel electrophoresis, as FISH probes for both metaphase chromosome spreads and 
      interphase nuclei. FISH chromosomal analysis using the three of the clones as 
      probes resulted in relatively simple hybridization patterns consistent with a 
      single homologous locus or two homoeologous loci. The fourth YAC probe resulted 
      in a diffuse hybridization pattern with signal on all metaphase chromosomes. We 
      conclude that YACs represent a valuable source of probes for chromosomal analysis 
      in soybean.
FAU - Zhu, T
AU  - Zhu T
AD  - Department of Biological Sciences, Northern Arizona University, Flagstaff 
      86011-5640, USA.
FAU - Shi, I
AU  - Shi I
FAU - Funke, R P
AU  - Funke RP
FAU - Gresshoff, P M
AU  - Gresshoff PM
FAU - Keim, P
AU  - Keim P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (DNA Primers)
SB  - IM
MH  - *Chromosomes, Artificial, Yeast
MH  - Cytogenetics/methods
MH  - DNA Primers
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase
MH  - Polymerase Chain Reaction/methods
MH  - Glycine max/*genetics
EDAT- 1996/09/25 00:00
MHDA- 1996/09/25 00:01
CRDT- 1996/09/25 00:00
PHST- 1996/09/25 00:00 [pubmed]
PHST- 1996/09/25 00:01 [medline]
PHST- 1996/09/25 00:00 [entrez]
AID - 10.1007/BF02173014 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1996 Sep 25;252(4):483-8. doi: 10.1007/BF02173014.