PMID- 8881030
OWN - NLM
STAT- MEDLINE
DCOM- 19961220
LR  - 20211203
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 45
IP  - 1
DP  - 1996
TI  - Cloning and chromosomal mapping of the mouse DNA-dependent protein kinase gene.
PG  - 1-5
AB  - Severe combined immune deficiency (scid) mice are assumed to have two types of 
      abnormalities: one is high radiosensitivity and the other is abnormal 
      recombination in immunoglobulin and T-cell receptor genes. The human chromosome 8 
      q1.1 region has an ability to complement the scid aberrations. Moreover, the 
      localization of the subunit DNA-dependent protein kinase [DNA-PKcs] participating 
      in DNA double-strand break repair in the same locus was clarified. In scid mouse 
      cells, the number of DNA-PKcs products and extent of DNA-PK activity remarkably 
      decrease. These observations gave rise to the assumption that DNA-PKcs is the 
      scid factor itself. In order to determine whether the DNA-PKcs gene is the scid 
      gene, we isolated the mouse DNA-PKcs gene and investigated its chromosomal locus 
      by fluorescence in situ hybridization (FISH). Consequently, it became clear that 
      the mouse DNA-PKcs gene existed in the centromeric region of mouse chromosome 16, 
      determined by cross-genetic study, as a scid locus. This finding strongly 
      suggests that mouse DNA-PKcs is the scid gene.
FAU - Hamatani, K
AU  - Hamatani K
AD  - Cell Biology Laboratory, Department of Radiobiology, Radiation Effects Research 
      Foundation, 8-6 Nakagawa 1 chome, Nagasaki 850, Japan.
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Araki, R
AU  - Araki R
FAU - Itoh, M
AU  - Itoh M
FAU - Abe, M
AU  - Abe M
LA  - eng
SI  - GENBANK/D83786
SI  - GENBANK/U34994
SI  - GENBANK/U35835
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
RN  - EC 2.7.11.1 (Prkdc protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA
MH  - DNA-Activated Protein Kinase
MH  - *DNA-Binding Proteins
MH  - Humans
MH  - Mice
MH  - Mice, SCID
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Proteins
MH  - Protein Serine-Threonine Kinases/*genetics
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/s002510050159 [doi]
PST - ppublish
SO  - Immunogenetics. 1996;45(1):1-5. doi: 10.1007/s002510050159.