PMID- 8882884
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 98
IP  - 5
DP  - 1996 Nov
TI  - Simultaneous detection of structural and numerical chromosome abnormalities in 
      sperm of healthy men by multicolor fluorescence in situ hybridization.
PG  - 608-15
AB  - Both structural and numerical chromosome aberrations in sperm represent important 
      categories of paternally transmitted genetic damage. Therefore, a new multiprobe 
      fluorescence in situ hybridization (FISH) method, using DNA probes for three 
      targets (centromere and telomere of chromosome 1, centromere of chromosome 8), 
      was developed to detect human sperm carrying three types of chromosomal defects: 
      (1) terminal duplications or deletions in chromosome 1p, (2) aneuploidy involving 
      chromosomes 1 or 8, and (3) diploidy. Baseline frequencies were determined for 
      three healthy donors who had been previously evaluated for sperm cytogenetics by 
      the human-sperm/hamster-oocyte cytogenetic technique (hamster technique). Among 
      approximately 120,000 sperm analyzed by the new FISH method, the average baseline 
      frequencies of sperm carrying telomeric duplications and deletions of 1p were 3.2 
      +/- 1.9 and 2.9 +/- 3.6 per 10(4), respectively. Diploid sperm was found in an 
      average frequency of 6.6 +/- 4.0 per 10(4). Average frequencies of disomic sperm 
      for chromosomes 1 or 8 were 1.7 +/- 2.2 and 1.9 +/- 2.3 per 10(4), respectively. 
      Inter-individual differences were observed for deletions of 1p but not for the 
      other sperm phenotypes. A good correlation was obtained between the frequencies 
      of sperm with structural chromosome aberrations detected with the new assay and 
      the frequency of sperm carrying premeiotic or meiotic cytogenetic damage detected 
      with the hamster technique. The observed levels of numerical aberrations with the 
      new FISH assay were within range of the baseline frequencies reported by the 
      hamster technique. The newly developed FISH assay has promising applications in 
      genetic, clinical, physiological and toxicological studies.
FAU - Van Hummelen, P
AU  - Van Hummelen P
AD  - Biology and Biotechnology Research Program, Lawrence Livermore National 
      Laboratory, CA 94550, USA. vanhummelen1@llnl.gov
FAU - Lowe, X R
AU  - Lowe XR
FAU - Wyrobek, A J
AU  - Wyrobek AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
SB  - IM
MH  - Adult
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 8
MH  - Cricetinae
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - *Spermatozoa
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/s004390050268 [doi]
PST - ppublish
SO  - Hum Genet. 1996 Nov;98(5):608-15. doi: 10.1007/s004390050268.