PMID- 8884848
OWN - NLM
STAT- MEDLINE
DCOM- 19970123
LR  - 20190905
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 13
IP  - 3
DP  - 1996 Oct
TI  - A rapid method to study heat shock protein 70-2 gene polymorphism in 
      insulin-dependent diabetes mellitus.
PG  - 268-72
AB  - To examine the role of DNA loci within the human leukocyte antigen (HLA) region 
      and insulin-dependent diabetes mellitus (IDDM), we studied fine mapping of 
      HSP70-2 gene. Polymerase chain reaction (PCR)-based genotyping was then developed 
      and applied to type HSP70-2 in 59 patients with IDDM and 83 unrelated controls 
      recruited from the inhabitants of northern Taiwan. Southern blot analysis 
      revealed a diallelic PstI polymorphism of the HSP 70-2 gene, i.e., 9.6- and 
      8.5-kb alleles. The polymorphic site was mapped in the intragenic PstI sequences 
      (nucleotides 1051-1056) of the HSP70-2 gene. PCR-based restriction fragment 
      length polymorphism studies revealed that the frequency of the 8.5-kb allele was 
      increased in IDDM (56.8%, vs. 40.4% in controls; p < 0.009), with a relative risk 
      of 1.93 (95% confidence interval = 1.20-3.11). The genotypic frequencies of 
      9.6/9.6, 9.6/8.5, and 8.5/8.5 were 17.0, 52.5, and 30.5% for IDDM were different 
      from those of controls (36.1, 47.0, and 16.9%, respectively; the homozygous 9.6/ 
      9.6 genotype was significantly decreased in the IDDM group, p < 0.02). In 
      conclusion, we provide a simple, rapid, and nonradioactive method for HSP70-2 
      genotyping. Our data confirmed that the 8.5-kb allele of HSP70-2 was associated 
      with IDDM susceptibility in the Taiwanese population.
FAU - Chuang, L M
AU  - Chuang LM
AD  - Department of Internal Medicine, College of Medicine, National Taiwan University, 
      Taipei, Taiwan, R.O.C.
FAU - Jou, T S
AU  - Jou TS
FAU - Wu, H P
AU  - Wu HP
FAU - Tai, T Y
AU  - Tai TY
FAU - Lin, B J
AU  - Lin BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - EC 3.1.21.4 (CTGCAG-specific type II deoxyribonucleases)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Blotting, Southern
MH  - Deoxyribonucleases, Type II Site-Specific
MH  - Diabetes Mellitus, Type 1/*genetics
MH  - Gene Frequency
MH  - Genotype
MH  - HSP70 Heat-Shock Proteins/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Restriction Fragment Length
MH  - Taiwan
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1097/00006676-199610000-00009 [doi]
PST - ppublish
SO  - Pancreas. 1996 Oct;13(3):268-72. doi: 10.1097/00006676-199610000-00009.