PMID- 8886003
OWN - NLM
STAT- MEDLINE
DCOM- 19961209
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 227
IP  - 3
DP  - 1996 Oct 23
TI  - Clonal analysis by chromosome 11 microsatellite-PCR of microdissected parathyroid 
      tumors from MEN 1 patients.
PG  - 736-42
AB  - Loss of heterozygosity (LOH) at chromosome 11q13 loci has been described in the 
      majority of larger parathyroid tumors from patients affected by Multiple 
      Endocrine Neoplasia type 1 (MEN 1) syndrome. Since classical histology of the 
      whole parathyroid gland does not permit a clear morpho-genetic correlation, the 
      clonal composition of abnormal parathyroid tissue was analyzed in DNA obtained 
      from single nodules and non-nodular areas within MEN 1 parathyroid lesions. 
      Microdissected sections were analyzed by chromosome 11q13 microsatellite-PCR for 
      LOH and by patterns of X-inactivation. We detected LOH for chromosome 11q13 loci 
      in at least one microdissected area for each familial MEN 1 patient, but not in 
      the single sporadic case. X-inactivation pattern of two "clonal" tumors exhibited 
      a polyclonal cell composition of these microdissected samples, indicating the 
      existence of a genetic heterogeneity in MEN 1 parathyroid microareas exhibiting a 
      "clonal" pattern for allelic losses.
FAU - Morelli, A
AU  - Morelli A
AD  - Department of Clinical Physiopathology, University of Florence, Italy.
FAU - Falchetti, A
AU  - Falchetti A
FAU - Amorosi, A
AU  - Amorosi A
FAU - Tonelli, F
AU  - Tonelli F
FAU - Bearzi, I
AU  - Bearzi I
FAU - Ranaldi, R
AU  - Ranaldi R
FAU - Tomassetti, P
AU  - Tomassetti P
FAU - Brandi, M L
AU  - Brandi ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chromosome Deletion
MH  - *Chromosomes, Human, Pair 11
MH  - DNA, Satellite
MH  - Dosage Compensation, Genetic
MH  - Female
MH  - Heterozygote
MH  - Humans
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Mosaicism
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Parathyroid Neoplasms/*genetics
MH  - Polymerase Chain Reaction
EDAT- 1996/10/23 00:00
MHDA- 2000/03/29 09:00
CRDT- 1996/10/23 00:00
PHST- 1996/10/23 00:00 [pubmed]
PHST- 2000/03/29 09:00 [medline]
PHST- 1996/10/23 00:00 [entrez]
AID - S0006-291X(96)91578-8 [pii]
AID - 10.1006/bbrc.1996.1578 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1996 Oct 23;227(3):736-42. doi: 
      10.1006/bbrc.1996.1578.