PMID- 8887747
OWN - NLM
STAT- MEDLINE
DCOM- 19970220
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 495 ( Pt 2)
IP  - Pt 2
DP  - 1996 Sep 1
TI  - Differentiation of the human monocytic cell line U937 results in an upregulation 
      of the calcium release-activated current, ICRAC.
PG  - 331-8
AB  - 1. Single cell fura-2 fluorescence measurements and whole-cell patch clamp 
      recordings were used to investigate the effects of macrophage-like 
      differentiation, induced by dibutyryl cAMP (dbcAMP), on Ca2+ influx triggered by 
      Ca2+ store depletion in the human monocytic cell line, U937. 2. In differentiated 
      cells, the rise in intracellular [Ca2+] following store depletion by thapsigargin 
      (TG) in nominally Ca(2+)-free solution was 94% greater and the [Ca2+]i rise on 
      subsequent re-addition of external Ca2+ (2 mM) was 292% greater than in 
      undifferentiated cells. 3. Under conditions where [Ca2+]i was buffered by BAPTA, 
      TG-induced store depletion failed to activate a detectable inward Ca2+ current in 
      undifferentiated U937 cells. Under identical conditions, store depletion of 
      differentiated U937 cells generated an inwardly rectifying Ca(2+)-selective 
      current which showed no reversal from -140 to +30 mV and was blocked by 1 microM 
      external La3+; characteristics of the calcium release-activated Ca2+ current 
      (ICRAC) identified in other cells. 4. We conclude that U937 cells show a 
      differentiation-dependent upregulation of a store-mediated Ca2+ entry pathway, 
      identified as ICRAC, which is not correlated with the small associated increase 
      in the size of TG-sensitive Ca2+ pools.
FAU - Floto, R A
AU  - Floto RA
AD  - Physiological Laboratory, University of Cambridge, UK. af@mole.bio.cam.ac.uk
FAU - Mahaut-Smith, M P
AU  - Mahaut-Smith MP
FAU - Allen, J M
AU  - Allen JM
FAU - Somasundaram, B
AU  - Somasundaram B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indicators and Reagents)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Bucladesine/pharmacology
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Channels/drug effects/*metabolism
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Line
MH  - Cell Membrane Permeability/drug effects
MH  - Electrophysiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Dyes
MH  - Fura-2
MH  - Humans
MH  - Indicators and Reagents
MH  - Membrane Potentials/physiology
MH  - Monocytes/drug effects/*physiology
MH  - Patch-Clamp Techniques
MH  - Thapsigargin/pharmacology
MH  - Up-Regulation/drug effects/*physiology
PMC - PMC1160795
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
PMCR- 1996/09/01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PHST- 1996/09/01 00:00 [pmc-release]
AID - 10.1113/jphysiol.1996.sp021597 [doi]
PST - ppublish
SO  - J Physiol. 1996 Sep 1;495 ( Pt 2)(Pt 2):331-8. doi: 
      10.1113/jphysiol.1996.sp021597.