PMID- 8887781
OWN - NLM
STAT- MEDLINE
DCOM- 19970219
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 495 ( Pt 3)
IP  - Pt 3
DP  - 1996 Sep 15
TI  - Ryanodine produces a low frequency stimulation-induced NMDA receptor-independent 
      long-term potentiation in the rat dentate gyrus in vitro.
PG  - 755-67
AB  - 1. The induction of long-term potentiation (LTP) was investigated in the rat 
      dentate gyrus in the presence of ryanodine, an agent which is known to 
      selectively bind to the ryanodine receptor (RyR) Ca2+ channels which regulate 
      Ca2+ release from intracellular Ca2+ stores. 2. In control media, high frequency 
      stimulation (HFS) induced LTP, and prolonged low frequency stimulation (LFS) 
      induced long-term depression (LTD), of field excitatory postsynaptic potentials 
      (EPSPs) and patch clamped excitatory postsynaptic currents (EPSCs). 3. In the 
      presence of ryanodine, at a threshold concentration of about 1 microM, 
      HFS-induced LTP was inhibited, whereas LFS (5 Hz, 900 pulses) now induced LTP. 4. 
      The N-methyl-D-aspartate receptor (NMDAR) antagonist 
      D-2-amino-phosphonopentanoate (D-AP5), at both 50 and 200 microM, did not prevent 
      the induction of LTP by 5 Hz LFS in the presence of ryanodine. This demonstrates 
      the NMDAR independence of LTP induction in the presence of ryanodine. 
      Furthermore, D AP5 reversed the block of HFS-induced LTP by ryanodine. 5. The 
      induction of LTP by 5 Hz LFS in the presence of ryanodine was blocked by lowering 
      extracellular Ca2+, or by rapidly buffering intracellular Ca2+ to very low levels 
      with BAPTA. 6. The induction of LTP by 5 Hz LFS was inhibited by the L-type 
      voltage-gated Ca2+ channel blocker nifedipine, and also by Ni2+ a commonly used T 
      type voltage-gated Ca2+ channel blocker. 7. The 5 Hz LFS-induced LTP in the 
      presence of ryanodine was inhibited by the metabotropic glutamate receptor 
      (mGluR) antagonist (+)-alpha-methyl 4-carboxyphenylglycine (MCPG). 8. The 5 Hz 
      LFS-induced LTP in the presence of ryanodine was blocked by Ruthenium Red, an 
      agent known to block RyR channel opening, and also by thapsigargin, an agent 
      known to block-ATP-dependent Ca2+ uptake into endoplasmic reticulum. 9. The 
      results of the present studies emphasize the importance of intracellular Ca2+ 
      stores in the induction of LTP.
FAU - Wang, Y
AU  - Wang Y
AD  - Department of Physiology, Trinity College, Dublin, Ireland.
FAU - Wu, J
AU  - Wu J
FAU - Rowan, M J
AU  - Rowan MJ
FAU - Anwyl, R
AU  - Anwyl R
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (Chelating Agents)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Muscle Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Ryanodine Receptor Calcium Release Channel)
RN  - 11103-72-3 (Ruthenium Red)
RN  - 15662-33-6 (Ryanodine)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - 7OV03QG267 (Nickel)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/pharmacology
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Channels/drug effects/metabolism
MH  - Chelating Agents/pharmacology
MH  - Dentate Gyrus/*drug effects/*physiology
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Electric Stimulation
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - In Vitro Techniques
MH  - Long-Term Potentiation/*drug effects
MH  - Muscle Proteins/drug effects/metabolism
MH  - Nickel/pharmacology
MH  - Nifedipine/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Ruthenium Red/pharmacology
MH  - Ryanodine/*pharmacology
MH  - Ryanodine Receptor Calcium Release Channel
MH  - Thapsigargin/pharmacology
PMC - PMC1160780
EDAT- 1996/09/15 00:00
MHDA- 1996/09/15 00:01
PMCR- 1996/09/15
CRDT- 1996/09/15 00:00
PHST- 1996/09/15 00:00 [pubmed]
PHST- 1996/09/15 00:01 [medline]
PHST- 1996/09/15 00:00 [entrez]
PHST- 1996/09/15 00:00 [pmc-release]
AID - 10.1113/jphysiol.1996.sp021631 [doi]
PST - ppublish
SO  - J Physiol. 1996 Sep 15;495 ( Pt 3)(Pt 3):755-67. doi: 
      10.1113/jphysiol.1996.sp021631.