PMID- 8889243 OWN - NLM STAT- MEDLINE DCOM- 19970204 LR - 20191101 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 4 IP - 6 DP - 1996 Sep TI - Human chromosome 3: high-resolution fluorescence in situ hybridization mapping of 40 unique NotI linking clones homologous to genes and cDNAs. PG - 443-7 AB - Forty new NotI linking clones representing sequence tagged sites (STSs) were mapped by fluorescence in situ hybridization (FISH) to different regions of human chromosome 3 (HSA3). Clone NL1-245, containing human aminoacylase 1, was localized to 3p21.2-p21.1. Our previous localization of the CLC-2 chloride channel protein gene was refined to 3q27. Clone NL2-316 most likely contains a translocon-associated protein gamma-subunit gene and was mapped to 3q23-q24. To our knowledge, this is the first time this gene has been mapped. One NotI linking clone (NL1-229) probably contains a new protein phosphatase gene. This clone was mapped to 3p25. Five NotI linking clones probably contain human expressed sequence tags (ESTs), as they possess sequences with a high level of identity (> 90%) to cDNA clones. Other clones show 56-85% homology to known mammalian and human genes with various functions, including oncogenes and tumour-suppressor genes. These clones might represent new genes. FAU - Protopopov, A I AU - Protopopov AI AD - Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia. FAU - Gizatullin, R Z AU - Gizatullin RZ FAU - Vorobieva, N V AU - Vorobieva NV FAU - Protopopova, M V AU - Protopopova MV FAU - Kiss, C AU - Kiss C FAU - Kashuba, V I AU - Kashuba VI FAU - Klein, G AU - Klein G FAU - Kisselev, L L AU - Kisselev LL FAU - Graphodatsky, A S AU - Graphodatsky AS FAU - Zabarovsky, E R AU - Zabarovsky ER LA - eng SI - GENBANK/AJ007298 SI - GENBANK/AJ007299 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 RN - 0 (DNA, Complementary) RN - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific) RN - EC 3.1.21.4 (GCGGCCGC-specific type II deoxyribonucleases) SB - IM MH - Animals MH - Base Sequence MH - Chromosome Mapping/methods MH - Chromosomes, Human, Pair 3/*chemistry MH - Cloning, Molecular MH - DNA, Complementary/chemistry MH - Deoxyribonucleases, Type II Site-Specific/*metabolism MH - Humans MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Rabbits MH - Rats MH - *Sequence Tagged Sites EDAT- 1996/09/01 00:00 MHDA- 1996/09/01 00:01 CRDT- 1996/09/01 00:00 PHST- 1996/09/01 00:00 [pubmed] PHST- 1996/09/01 00:01 [medline] PHST- 1996/09/01 00:00 [entrez] AID - 10.1007/BF02265051 [doi] PST - ppublish SO - Chromosome Res. 1996 Sep;4(6):443-7. doi: 10.1007/BF02265051.