PMID- 8889675
OWN - NLM
STAT- MEDLINE
DCOM- 19970130
LR  - 20190512
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 20
IP  - 6
DP  - 1996 Oct
TI  - Detection of methamphetamine in sweat by EIA and GC-MS.
PG  - 398-403
AB  - Sweat was collected with the PharmChekTM sweat patch and drugs were eluted from 
      the collection pad of the patch. A solid phase, enzyme immunoassay using 
      microtiter plates was modified for analysis of methamphetamine in sweat. After 
      methamphetamine administration, sweat contains primarily parent methamphetamine. 
      The immunoassay was determined to have crossreactivity relative to 100% for the 
      methamphetamine (MA) calibrators; to 144% for methylenedioxymethamphetamine 
      (MDMA); to 30% for d-amphetamine; to 21% for methylenedioxyamphetamine (MDA); and 
      to 8% for I-methamphetamine. The optimum cutoff concentration for this modified 
      assay was determined by receiver operating characteristic analysis to be 10 ng/mL 
      amphetamine equivalents. At this cutoff concentration the assay had a diagnostic 
      sensitivity of 84.5% and a diagnostic specificity of 93.2% versus gas 
      chromatography-mass spectrometry (GC-MS). The positive predictive value at a 
      prevalence of 50% was 86%. The intra-assay precision at 10 ng/mL was 9.9% 
      (coefficient of variation, CV) and the interassay CV was 13%. Analysis of spiked 
      patches at plus or minus 25 and 50% around the cutoff gave a percent positive 
      threshold of approximately 50% at a cutoff of 10 ng/mL and a 95% confidence level 
      for a positive result by the EIA between 15 and 20 ng/mL. Of 18 potential 
      adulterants that might be injected into or under the patch, two (tile cleaner and 
      cough syrup) caused a false-positive response by immunoassay. All results were 
      confirmed by GC-MS. The clinical sensitivity and specificity of the overall 
      analysis system (sweat collection and analysis) were 85 and 100%, respectively, 
      using known methamphetamine dosing of volunteers (10, 20, and 25 mg) as the 
      reference standard.
FAU - Fay, J
AU  - Fay J
AD  - STC Diagnostics, Bethlehem, Pennsylvania, USA.
FAU - Fogerson, R
AU  - Fogerson R
FAU - Schoendorfer, D
AU  - Schoendorfer D
FAU - Niedbala, R S
AU  - Niedbala RS
FAU - Spiehler, V
AU  - Spiehler V
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 44RAL3456C (Methamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
CIN - ACP J Club. 1997 May-Jun;126(3):76
MH  - Calibration
MH  - Cross Reactions
MH  - Drug Interactions
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Methamphetamine/analysis/*metabolism
MH  - N-Methyl-3,4-methylenedioxyamphetamine/analysis/*metabolism
MH  - ROC Curve
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sweat/*chemistry/metabolism
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1093/jat/20.6.398 [doi]
PST - ppublish
SO  - J Anal Toxicol. 1996 Oct;20(6):398-403. doi: 10.1093/jat/20.6.398.