PMID- 8890918
OWN - NLM
STAT- MEDLINE
DCOM- 19961127
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 59
IP  - 17
DP  - 1996
TI  - Intra-adrenal 11 beta-hydroxysteroid dehydrogenase plays a role in the regulation 
      of corticosteroid secretion: an in vitro study in the rat.
PG  - 1401-6
AB  - The expression of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) has been 
      demonstrated in the adrenal glands, but until now little attention has been paid 
      on its possible physiologic action. In-situ perfused rat adrenals released under 
      basal conditions, in addition to mineralo- and glucocorticoids, notable amounts 
      of 11-dehydrocorticosterone (DH-B), the inactive form to which corticosterone 
      (the main glucocorticoid in rodents) is converted by 11 beta-HSD. The addition to 
      the perfusion medium of glycyrrhetinic acid, a specific inhibitor of 11 beta-HSD, 
      strongly reduced DH-B release and simultaneously evoked a moderate rise in both 
      mineralo- and glucocorticoid output. The bolus administration of ACTH strikingly 
      enhanced mineralo- and glucocorticoid secretion, but it significantly depressed 
      DH-B release Rat adrenal microsome preparations possessed 11 beta-HSD activity, 
      that was inhibited by glycyrrhetinic acid. Conversely, ACTH was without any 
      apparent effect, a finding indicating that the in vivo observed ACTH-induced 
      inhibition of adrenal 11 beta-HSD activity is mediated by an indirect mechanism 
      whose elucidation requires further investigation. In conclusion, our present 
      findings suggest that adrenal 11 beta-HSD plays a role in the regulation of 
      steroid secretion in rats under both basal and stimulated conditions.
FAU - Musajo, F
AU  - Musajo F
AD  - Department of Anatomy, University of Padua, Italy.
FAU - Neri, G
AU  - Neri G
FAU - Tortorella, C
AU  - Tortorella C
FAU - Mazzocchi, G
AU  - Mazzocchi G
FAU - Nussdorfer, G G
AU  - Nussdorfer GG
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Adrenal Cortex Hormones)
RN  - 4964P6T9RB (Aldosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Adrenal Cortex Hormones/*metabolism
MH  - Adrenal Glands/*enzymology/metabolism
MH  - Aldosterone/metabolism
MH  - Animals
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Progesterone/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 0024320596004675 [pii]
AID - 10.1016/0024-3205(96)00467-5 [doi]
PST - ppublish
SO  - Life Sci. 1996;59(17):1401-6. doi: 10.1016/0024-3205(96)00467-5.