PMID- 8891288
OWN - NLM
STAT- MEDLINE
DCOM- 19970206
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 732
IP  - 1-2
DP  - 1996 Sep 2
TI  - Ultrastructural localization of the kainate selective glutamate receptor in 
      noradrenergic perikarya and dendrites of the nucleus locus coeruleus in the rat 
      brain.
PG  - 223-31
AB  - To determine the regional and cellular distribution of the kainate receptor 
      subtype in the LC, we combined immunoperoxidase labeling of an antibody which 
      recognized the three identified members of the kainate receptor subunit class, 
      with immunogold-silver localization of tyrosine hydroxylase (TH). Light 
      microscopy showed that kainate receptor-like (KAr-li) immunoreactivity was dense 
      throughout the LC. Electron microscopy revealed that KAr-li immunoreactivity was 
      mainly located at synaptic and extrasynaptic portions of the plasmalemma in 
      dendrites and somata, many of which also contained gold-silver labeling for TH. 
      Unlabeled axon terminals often formed asymmetric (excitatory-type) synaptic 
      contacts with postsynaptic dendrites containing KAr-li immunolabeling. In 
      perikarya, KAr-li immunoreactivity was often associated with subcellular 
      organelles including the Golgi apparatus and endoplasmic reticulum. Axon 
      terminals were infrequently immunolabeled for KAr-li in the LC. These results 
      provide the first direct ultrastructural evidence that the excitatory amino acid, 
      glutamate, may locally modulate noradrenergic neurons in the LC through 
      kainate-type receptors located at postsynaptic and extrasynaptic cellular sites.
FAU - Van Bockstaele, E J
AU  - Van Bockstaele EJ
AD  - Department of Neurology and Neuroscience, Cornell University Medical College, New 
      York, NY 10021, USA.
FAU - Colago, E E
AU  - Colago EE
LA  - eng
GR  - NIMH 18974/MH/NIMH NIH HHS/United States
GR  - R29 DA09082/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Receptors, Kainic Acid)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/*ultrastructure
MH  - Dendrites/metabolism/*ultrastructure
MH  - Immunoenzyme Techniques
MH  - Locus Coeruleus/metabolism/*ultrastructure
MH  - Male
MH  - Microscopy, Immunoelectron
MH  - Neurons/metabolism/*ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Kainic Acid/*analysis/metabolism
EDAT- 1996/09/02 00:00
MHDA- 1996/09/02 00:01
CRDT- 1996/09/02 00:00
PHST- 1996/09/02 00:00 [pubmed]
PHST- 1996/09/02 00:01 [medline]
PHST- 1996/09/02 00:00 [entrez]
AID - 0006-8993(96)00569-0 [pii]
AID - 10.1016/0006-8993(96)00569-0 [doi]
PST - ppublish
SO  - Brain Res. 1996 Sep 2;732(1-2):223-31. doi: 10.1016/0006-8993(96)00569-0.