PMID- 8891348
OWN - NLM
STAT- MEDLINE
DCOM- 19970203
LR  - 20081121
IS  - 0145-5680 (Print)
IS  - 0145-5680 (Linking)
VI  - 42
IP  - 6
DP  - 1996 Sep
TI  - Activation of the transcription from the human immunodeficiency virus type 1 
      (HIV-1) long terminal repeat by autologous and heterologous cell-to-cell contact.
PG  - 811-23
AB  - Recent studies have demonstrated that the activation of HIV-1 provirus and of the 
      Long Terminal Repeat of HIV-1 (HIV-1-LTR) may occur upon cell-to-cell contact 
      between peripheral blood lymphocytes (PBLs) and infected or transfected (HT29) 
      human colonic carcinoma cells. Using transient or stable transfections, we 
      ascertained that the HIV-1 LTR was up-regulated by cell-to-cell contact in 
      various cell lines. The degree of cell-to-cell contact responsiveness was cell 
      type dependent. In contrast, in transient transfection, the HIV-1-LTR was 
      strongly induced by Tat expression in all cell types tested. This indicates that 
      there are differences in the induction mechanism for these two stimuli, even 
      though Tat protein has been previously reported to induce cell adhesion. Except 
      for the PBLs/transfected cells interactions, the results also demonstrate that 
      the cell-to-cell contact-induced HIV-1-LTR activation was highest after contact 
      between autologous as compared to heterologous cells. Previous experiments have 
      shown that, in HT29 cells, cell-to-cell contact activation of the HIV-1-LTR 
      involved the NF-kappa B tandem binding site and activates DNA binding of the 
      nuclear factor NF-kappa B. In this work, we show that in a stably transfected 
      cell line, the principal enhancer element was also involved in the HIV-1-LTR 
      cell-to-cell contact activation. On the other hand, in the HT29 cell line, the 
      NF-kappa B binding appeared to involve the RGD motif of the cell-binding domain, 
      which indicates that a post-integrin receptor event could be implicated.
FAU - Faure, E
AU  - Faure E
AD  - Institut de Chimie Biologique, Universite de Provence, Marseille, France.
FAU - Lecine, P
AU  - Lecine P
FAU - Imbert, J
AU  - Imbert J
FAU - Champion, S
AU  - Champion S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
SB  - IM
MH  - Cell Communication/*genetics
MH  - Cells, Cultured
MH  - Colonic Neoplasms/genetics/pathology
MH  - HIV Long Terminal Repeat/*genetics
MH  - Humans
MH  - Lymphocytes/pathology
MH  - Transcriptional Activation
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PST - ppublish
SO  - Cell Mol Biol (Noisy-le-grand). 1996 Sep;42(6):811-23.