PMID- 8891442
OWN - NLM
STAT- MEDLINE
DCOM- 19970124
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 25
IP  - 2
DP  - 1996 Oct 1
TI  - Interlaboratory variability in fluorescence in situ hybridization analysis. The 
      NCI Bladder Tumor Marker Network.
PG  - 125-32
AB  - Reliable interpretation of fluorescence in situ hybridization (FISH) data, 
      especially data that have been generated in more than one laboratory, requires 
      knowledge of the sources of variability inherent in FISH analysis. Possible 
      sources of variation may derive from differences in sample preparation, probes 
      used, intrasample heterogeneity, hybridization protocols, counting criteria 
      within and between scorers, fluorescence microscopes, and filters. This study 
      characterized the relative weight of some of these factors in order to determine 
      the degree to which FISH results are comparable between laboratories. We used a 
      hierarchical partitioned chi 2 analysis to measure sources of variation. We found 
      that replicate counts varied no more than expected based on counting statistics 
      (i.e., multinomial variation). However, with replicate hybridizations done in two 
      separate laboratories, the variability increased significantly. Thus, care must 
      be taken when interpreting FISH data that are derived from more than one 
      institution. Previously agreed upon counting criteria as well as standardized 
      FISH hybridization protocols may decrease this variability.
FAU - Moore, D H 2nd
AU  - Moore DH 2nd
AD  - Department of Epidemiology, University of California San Francisco 94143-0808, 
      USA. Moore@dmc.ucsf.edu
FAU - Epstein, L
AU  - Epstein L
FAU - Reeder, J
AU  - Reeder J
FAU - Wheeless, L
AU  - Wheeless L
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA47537/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
SB  - IM
MH  - Chi-Square Distribution
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 9
MH  - Data Interpretation, Statistical
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/standards
MH  - Laboratories/standards
MH  - Urinary Bladder Neoplasms/genetics/pathology
EDAT- 1996/10/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1002/cyto.990250202 [doi]
PST - ppublish
SO  - Cytometry. 1996 Oct 1;25(2):125-32. doi: 10.1002/cyto.990250202.