PMID- 8892671
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 10
IP  - 11
DP  - 1996 Nov
TI  - Correlation of trisomy 12 with proliferating cells by combined 
      immunocytochemistry and fluorescence in situ hybridization in chronic lymphocytic 
      leukemia.
PG  - 1705-11
AB  - Conventional G-banding and fluorescence in situ hybridization (FISH) were 
      performed on peripheral blood samples of 340 consecutive untreated cases of 
      chronic lymphocytic leukemia (CLL) for the detection of trisomy 12 and other 
      chromosome abnormalities. These findings were correlated with the proliferative 
      activity of CLL lymphocytes assessed by the monoclonal antibody Ki-67. 
      Cytogenetic analysis displayed a normal karyotype in 131 (38.5%) cases, trisomy 
      12 in 68 (20%), 31 by G-banding and an additional 37 cases by FISH, other clonal 
      abnormalities in 47 (14%), and no metaphases in 94 (27.5%). The percentage of 
      Ki-67-positive cells was significantly higher in cases with trisomy 12 (4.1 +/- 
      4.48) than in cases with a normal karyotype (1.5 +/- 2.0), those with other 
      clonal abnormalities (1.35 +/- 1.37) and cases with no metaphases (1.14 +/- 1.6) 
      (P< 0.0001). Cases with trisomy 12 were associated with more advanced clinical 
      stage, atypical morphology and a higher percentage of Ki-67+ve cells than cases 
      lacking trisomy 12 (P< 0.0001). Although there was no direct correlation between 
      the percentage of trisomic and proliferating cells, the combination of 
      immunocytochemistry and FISH showed that most Ki-67-positive cells were trisomic 
      for chromosome 12. Our results suggest that the association of trisomy 12 with a 
      higher proliferative activity supports the view that this abnormality is a 
      secondary event associated with disease progression in CLL.
FAU - Garcia-Marco, J A
AU  - Garcia-Marco JA
AD  - Academic Department of Haematology and Cytogenetics, The Royal Marsden Hospital, 
      London, UK.
FAU - Price, C M
AU  - Price CM
FAU - Ellis, J
AU  - Ellis J
FAU - Morey, M
AU  - Morey M
FAU - Matutes, E
AU  - Matutes E
FAU - Lens, D
AU  - Lens D
FAU - Colman, S
AU  - Colman S
FAU - Catovsky, D
AU  - Catovsky D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Division
MH  - *Chromosomes, Human, Pair 12
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Ki-67 Antigen
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/blood/*genetics/pathology
MH  - Lymphocytes/*pathology
MH  - Male
MH  - Middle Aged
MH  - *Trisomy
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1996 Nov;10(11):1705-11.