PMID- 8892963
OWN - NLM
STAT- MEDLINE
DCOM- 19961224
LR  - 20181130
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 228
IP  - 1
DP  - 1996 Oct 10
TI  - 9-cis-retinoic acid induces neuronal differentiation of retinoic 
      acid-nonresponsive embryonal carcinoma cells.
PG  - 1-7
AB  - P19 mouse embryonal carcinoma cells differentiate into neurons and glial cells 
      when treated with retinoic acid. In contrast, a subline of the P19 cells, RAC65, 
      is known to show little sign of differentiation with the treatment. We treated 
      the two embryonal carcinoma (EC) cell lines with 9-cis-retinoic acid and 
      investigated its neuron-inducing activity. In P19 cells, 9-cis-retinoic acid 
      showed an activity equal to that of all-trans-retinoic acid. However in RAC65 
      cells, 9-cis-retinoic acid induced neurons 10-fold more effectively than 
      all-trans-retinoic acid. The order in which various retinoids appeared in P19 
      cells corresponded to that of retinoic acid receptors, and the order in RAC65 
      cells to that of retinoid X receptors (RXRs). Furthermore we found that the 
      down-regulation of retinoid X receptor-gamma mRNA expression was associated with 
      neuronal differentiation in both embryonal carcinoma cell lines. In addition, a 
      synthetic RXR-selective retinoid induced neurons from both EC cells. Our findings 
      support an intriguing possibility that the 9-cis-retinoic acid/retinoid X 
      receptor system may play an important role in neural differentiation.
FAU - Yokota, Y
AU  - Yokota Y
AD  - Institute of Molecular Embryology and Genetics, Kumamoto University School of 
      Medicine, Japan.
FAU - Ohkubo, H
AU  - Ohkubo H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Retinoids)
RN  - 0 (Tetrahydronaphthalenes)
RN  - 0 (Transcription Factors)
RN  - 5688UTC01R (Tretinoin)
RN  - A61RXM4375 (Bexarotene)
SB  - IM
MH  - Animals
MH  - Bexarotene
MH  - Carcinoma, Embryonal/genetics/metabolism/pathology
MH  - Cell Differentiation/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation
MH  - Mice
MH  - Neurons/cytology/*drug effects/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Retinoic Acid/agonists/genetics/metabolism
MH  - Retinoid X Receptors
MH  - Retinoids/metabolism/pharmacology
MH  - Stereoisomerism
MH  - Tetrahydronaphthalenes/pharmacology
MH  - Transcription Factors/agonists/genetics/metabolism
MH  - Tretinoin/administration & dosage/chemistry/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1996/10/10 00:00
MHDA- 1996/10/10 00:01
CRDT- 1996/10/10 00:00
PHST- 1996/10/10 00:00 [pubmed]
PHST- 1996/10/10 00:01 [medline]
PHST- 1996/10/10 00:00 [entrez]
AID - S0014-4827(96)90291-8 [pii]
AID - 10.1006/excr.1996.0291 [doi]
PST - ppublish
SO  - Exp Cell Res. 1996 Oct 10;228(1):1-7. doi: 10.1006/excr.1996.0291.