PMID- 8893809 OWN - NLM STAT- MEDLINE DCOM- 19961206 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 74 IP - 1-2 DP - 1996 TI - Cloning, expression and chromosome mapping of adducin-like 70 (ADDL), a human cDNA highly homologous to human erythrocyte adducin. PG - 90-5 AB - From a human fetal-brain cDNA library we isolated a novel human cDNA, termed human adducin-like 70 (gene symbol ADDL), whose predicted amino acid sequence showed a high degree of homology to adducins. This cDNA clone (ADDL), which contained an open reading frame of 2,022 nucleotides encoding 674 amino acids, revealed 54%, 53%, and 59% identity in predicted amino acid sequence with alpha and beta components of human adducin and rat adducin 63, respectively. Human adducin-like 70 is likely to play an important role in the skeletal organization of the cell membrane. Northern blot analysis indicated ubiquitous expression of this gene in adult human tissues. We localized the gene to chromosome bands 10q24.2-->q24.3 by fluorescence in situ hybridization (FISH). FAU - Katagiri, T AU - Katagiri T AD - Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan. FAU - Ozaki, K AU - Ozaki K FAU - Fujiwara, T AU - Fujiwara T FAU - Shimizu, F AU - Shimizu F FAU - Kawai, A AU - Kawai A FAU - Okuno, S AU - Okuno S FAU - Suzuki, M AU - Suzuki M FAU - Nakamura, Y AU - Nakamura Y FAU - Takahashi, E AU - Takahashi E FAU - Hirai, Y AU - Hirai Y LA - eng SI - GENBANK/D67031 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 RN - 0 (ADD3 protein, human) RN - 0 (Calmodulin-Binding Proteins) RN - 0 (Cytoskeletal Proteins) RN - 0 (DNA, Complementary) RN - 0 (adducin) SB - IM MH - Adult MH - Base Sequence MH - Blotting, Northern MH - Calmodulin-Binding Proteins/*genetics MH - *Chromosome Mapping MH - *Chromosomes, Human, Pair 10 MH - Cloning, Molecular MH - Cosmids MH - Cytoskeletal Proteins/genetics MH - DNA, Complementary/genetics MH - Erythrocytes/*chemistry MH - Fetus MH - Gene Expression/genetics MH - Gene Library MH - Humans MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid EDAT- 1996/01/01 00:00 MHDA- 1996/01/01 00:01 CRDT- 1996/01/01 00:00 PHST- 1996/01/01 00:00 [pubmed] PHST- 1996/01/01 00:01 [medline] PHST- 1996/01/01 00:00 [entrez] AID - 10.1159/000134389 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1996;74(1-2):90-5. doi: 10.1159/000134389.