PMID- 8894085
OWN - NLM
STAT- MEDLINE
DCOM- 19970130
LR  - 20190815
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 17
IP  - 20
DP  - 1996 Oct
TI  - Histomorphological reaction of bone to different concentrations of phagocytosable 
      particles of high-density polyethylene and Ti-6Al-4V alloy in vivo.
PG  - 1943-7
AB  - Wear debris has been implicated in the pathogenesis of loosening and osteolysis 
      of total joint replacements by stimulating a foreign body and chronic 
      inflammatory reaction capable of bone resorption. Whether increasing 
      concentrations of wear particles have an adverse biological effect on bone has 
      not been elucidated. We performed a histomorphological and semi-quantitative 
      morphometric analysis of the reaction of bone to different concentrations of 
      phagocytosable particles of high-density polyethylene (HDPE) and titanium 
      6-aluminium 4-vanadium alloy (Ti-6Al-4V) implanted in the rabbit tibia. The 
      Ti-6Al-4V particles had a diameter of 4.0 +/- 4.4 microns (mean +/- SD); the HDPE 
      particles averaged 4.7 +/- 2.1 microns. Suspensions of 10(6)-10(9) particles per 
      ml were mixed in saline, sterilized, and introduced through a drill hole into the 
      proximal tibia of 30 mature female rabbits. Controls included drilled, but 
      non-implantable limbs. The animals were killed at 16 weeks and histological 
      sections were made of the implant area. Histomorphological assessment was carried 
      out using an interactive image analysis system. The parameters assessed included 
      the presence of histiocytes, foreign body giant cells and inflammatory cells, the 
      location and number of particles, the presence of haematopoeitic elements, fat or 
      necrosis of the marrow, whether healing of the cortical window had taken place, 
      and whether there was evidence of formation or resorption of bone by the 
      periosteum, cortex and marrow. A semi-quantitative rating system was employed. 
      Phagocytosable particles of Ti-6Al-4V and HDPE, in concentrations of 10(6)-10(9) 
      particles per ml, evoked a histiocytic reaction without extensive fibrosis, 
      necrosis or granuloma formation. This reaction occurred without disturbing the 
      normal repair processes of bone formation and resorption to the surgical insult. 
      A clear dose-response effect on the histological parameters assessed in this 
      study was not noted. Using the present model, by 16 weeks, a similar "one time' 
      particle load could be accommodated. The ongoing generation of particulate debris 
      over a more extended period of time might be necessary before the remodelling 
      processes of bone would be disturbed.
FAU - Goodman, S B
AU  - Goodman SB
AD  - Stanford University Medical Center, Division of Orthopaedic Surgery, CA 
      94305-5326, USA.
FAU - Davidson, J A
AU  - Davidson JA
FAU - Song, Y
AU  - Song Y
FAU - Martial, N
AU  - Martial N
FAU - Fornasier, V L
AU  - Fornasier VL
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Alloys)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polyethylenes)
RN  - 00J9J9XKDE (Vanadium)
RN  - CPD4NFA903 (Aluminum)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Alloys
MH  - Aluminum/adverse effects/*metabolism
MH  - Animals
MH  - Biocompatible Materials
MH  - Bone Development/drug effects
MH  - Bone Marrow/drug effects/metabolism
MH  - Bone Resorption/chemically induced
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Molecular Weight
MH  - Periosteum/drug effects/metabolism
MH  - Phagocytosis/*drug effects
MH  - Polyethylenes/adverse effects/*metabolism
MH  - Prostheses and Implants/adverse effects
MH  - Rabbits
MH  - Tibia/metabolism
MH  - Titanium/adverse effects/*metabolism
MH  - Vanadium/adverse effects/*metabolism
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 0142961296000130 [pii]
AID - 10.1016/0142-9612(96)00013-0 [doi]
PST - ppublish
SO  - Biomaterials. 1996 Oct;17(20):1943-7. doi: 10.1016/0142-9612(96)00013-0.