PMID- 8896561
OWN - NLM
STAT- MEDLINE
DCOM- 19961216
LR  - 20211203
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 14
IP  - 3
DP  - 1996 Nov
TI  - Multicolour spectral karyotyping of mouse chromosomes.
PG  - 312-5
AB  - Murine models of human carcinogenesis are exceedingly valuable tools to 
      understand genetic mechanisms of neoplastic growth. The identification of 
      recurrent chromosomal rearrangements by cytogenetic techniques serves as an 
      initial screening test for tumour specific aberrations. In murine models of human 
      carcinogenesis, however, karyotype analysis is technically demanding because 
      mouse chromosomes are acrocentric and of similar size. Fluorescence in situ 
      hybridization (FISH) with mouse chromosome specific painting probes can 
      complement conventional banding analysis. Although sensitive and specific, FISH 
      analyses are restricted to the visualization of only a few mouse chromosomes at a 
      time. Here we apply a novel imaging technique that we developed recently for the 
      visualization of human chromosomes to the simultaneous discernment of all mouse 
      chromosomes. The approach is based on spectral imaging to measure 
      chromosome-specific spectra after FISH with differentially labelled mouse 
      chromosome painting probes. Utilizing a combination of Fourier spectroscopy, 
      CCD-imaging and conventional optical microscopy, spectral imaging allows 
      simultaneous measurement of the fluorescence emission spectrum at all sample 
      points. A spectrum-based classification algorithm has been adapted to karyotype 
      mouse chromosomes. We have applied spectral karyotyping (SKY) to chemically 
      induced plasmocytomas, mammary gland tumours from transgenic mice overexpressing 
      the c-myc oncogene and thymomas from mice deficient for the ataxia telangiectasia 
      (Atm) gene. Results from these analyses demonstrate the potential of SKY to 
      identify complex chromosomal aberrations in mouse models of human carcinogenesis.
FAU - Liyanage, M
AU  - Liyanage M
AD  - Diagnostic Development Branch, National Center for Human Genome Research, 
      National Institutes of Health, Bethesda, Maryland 20892-4470, USA.
FAU - Coleman, A
AU  - Coleman A
FAU - du Manoir, S
AU  - du Manoir S
FAU - Veldman, T
AU  - Veldman T
FAU - McCormack, S
AU  - McCormack S
FAU - Dickson, R B
AU  - Dickson RB
FAU - Barlow, C
AU  - Barlow C
FAU - Wynshaw-Boris, A
AU  - Wynshaw-Boris A
FAU - Janz, S
AU  - Janz S
FAU - Wienberg, J
AU  - Wienberg J
FAU - Ferguson-Smith, M A
AU  - Ferguson-Smith MA
FAU - Schrock, E
AU  - Schrock E
FAU - Ried, T
AU  - Ried T
LA  - eng
GR  - 1P50CA58185/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.11.1 (ATM protein, human)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - EC 2.7.11.1 (Atm protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Ataxia Telangiectasia Mutated Proteins
MH  - Cell Cycle Proteins
MH  - *Chromosome Aberrations
MH  - *Chromosomes
MH  - DNA-Binding Proteins
MH  - Disease Models, Animal
MH  - Genes, myc
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/*methods
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - Neoplasms/genetics
MH  - Plasmacytoma/genetics
MH  - *Protein Serine-Threonine Kinases
MH  - Proteins/genetics
MH  - Tumor Suppressor Proteins
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1038/ng1196-312 [doi]
PST - ppublish
SO  - Nat Genet. 1996 Nov;14(3):312-5. doi: 10.1038/ng1196-312.