PMID- 8896720
OWN - NLM
STAT- MEDLINE
DCOM- 19970206
LR  - 20190905
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 70
IP  - 11
DP  - 1996
TI  - Change of embryotoxic susceptibility to di-n-butyltin dichloride in cultured rat 
      embryos.
PG  - 742-8
AB  - Di-n-butylin dichloride (DBTCl), which is commonly used as heat and light 
      stabilizer for polyvinyl chloride (PVC) plastics, is a teratogen in vivo. In the 
      present study, the toxic effects were investigated of DBTCl on cultured rat 
      embryos during three different stages of organogenesis. Rat embryos explanted on 
      gestational day (GD) 8.5, GD 9.5, and GD 11.5 were cultured for 68, 46, and 48 h 
      and were exposed to a range of DBTCl concentrations for the first 24, 46, and the 
      last 46 h of culture, respectively. Significant decreases in the placental 
      diameter at > or = 10 ng/ml and in the number of somite pairs and the 
      morphological score at 30 ng/ml were noted in embryos cultured from GD 8.5. 
      Significant decreases in the yolk sac diameter and the crown-rump length at 100 
      ng/ml, in the number of somite pairs at > or = 50 ng/ml, and in the morphological 
      score at > or = 30 ng/ml were found in embryos cultured from GD 9.5. No adverse 
      effects on these parameters were detected in embryos cultured from GD 11.5 even 
      at 300 ng/ml. Dysmorphogenesis in embryos cultured from GD 8.5, GD 9.5, and GD 
      11.5 was observed at > or = 10, > or = 50, and 300 ng/ml, respectively. 
      Incomplete turning and craniofacial defects in embryos cultured from GD 8.5 and 
      GD 9.5 and defects of the forelimb buds and tail in embryos cultured from GD 11.5 
      were frequently observed. These results show that in vitro exposure to DBTCl 
      interferes with normal development of embryos during three different stages of 
      organogenesis and that susceptibility to the embryotoxicity, including the 
      dysmorphogenic potential of DBTCl, varies with developmental stage.
FAU - Ema, M
AU  - Ema M
AD  - National Institute of Health Sciences, Osaka, Japan.
FAU - Iwase, T
AU  - Iwase T
FAU - Iwase, Y
AU  - Iwase Y
FAU - Ohyama, N
AU  - Ohyama N
FAU - Ogawa, Y
AU  - Ogawa Y
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Organotin Compounds)
RN  - 0 (Teratogens)
RN  - J4AQN88R8P (dibutyldichlorotin)
SB  - IM
MH  - Animals
MH  - Disease Susceptibility
MH  - Embryo, Mammalian/*drug effects
MH  - Embryonic and Fetal Development/*drug effects
MH  - Female
MH  - Male
MH  - Organ Culture Techniques
MH  - Organotin Compounds/*toxicity
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Teratogens/*toxicity
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/s002040050335 [doi]
PST - ppublish
SO  - Arch Toxicol. 1996;70(11):742-8. doi: 10.1007/s002040050335.