PMID- 8897011
OWN - NLM
STAT- MEDLINE
DCOM- 19970224
LR  - 20220129
IS  - 0012-186X (Print)
IS  - 0012-186X (Linking)
VI  - 39
IP  - 10
DP  - 1996 Oct
TI  - Association of IA-2 autoantibodies with HLA DR4 phenotypes in IDDM.
PG  - 1223-6
AB  - Insulin, glutamate decarboxylase (GAD) and the protein tyrosine phosphatase-like 
      molecule IA-2 are major targets of humoral autoimmunity in insulin-dependent 
      diabetes mellitus (IDDM). These autoantibodies are heterogeneous with respect to 
      age and patient human leukocyte antigen (HLA) phenotype. We have previously 
      demonstrated that GAD and IA-2 antibodies potentially identify different subsets 
      of IDDM patients. The aim of this study was to determine whether GAD and IA-2 
      autoantibodies were associated with different HLA DR phenotypes. We studied 160 
      patients with IDDM onset before age 16 years. At disease onset serum was tested 
      for GAD and IA-2 antibodies by immunoprecipitation by in vitro-translated 
      35S-methionine labelled recombinant proteins. IA-2 antibodies were significantly 
      associated with HLA DR4: 67 (86%) of 78 patients with HLA DR4 vs 31 (38%) of 82 
      non-DR4 patients had IA-2 antibodies (Pc < 0.0001) and IA-2 antibody levels were 
      higher in patients with HLA DR4 (Pc < 0.0001). In contrast, GAD antibodies were 
      more prevalent (Pc < 0.05) and antibody levels highest (Pc < 0.01) in patients 
      with HLA DR3 phenotypes. These data provide further evidence that, in IDDM, 
      production and titre of major autoantibody specificities are associated with HLA 
      class II alleles.
FAU - Genovese, S
AU  - Genovese S
AD  - Department of Internal Medicine, Istituto Scientifico San Raffaele, University of 
      Milan, Italy.
FAU - Bonfanti, R
AU  - Bonfanti R
FAU - Bazzigaluppi, E
AU  - Bazzigaluppi E
FAU - Lampasona, V
AU  - Lampasona V
FAU - Benazzi, E
AU  - Benazzi E
FAU - Bosi, E
AU  - Bosi E
FAU - Chiumello, G
AU  - Chiumello G
FAU - Bonifacio, E
AU  - Bonifacio E
LA  - eng
GR  - E.0587/TI_/Telethon/Italy
GR  - TGT06S01/TI_/Telethon/Italy
PT  - Journal Article
PL  - Germany
TA  - Diabetologia
JT  - Diabetologia
JID - 0006777
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (HLA-DR3 Antigen)
RN  - 0 (HLA-DR4 Antigen)
RN  - 0 (Membrane Proteins)
RN  - EC 3.1.3.48 (PTPRN protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 8)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
SB  - IM
MH  - Alleles
MH  - Autoantibodies/*blood
MH  - Autoantigens/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Mellitus, Type 1/genetics/*immunology
MH  - Glutamate Decarboxylase/*immunology
MH  - HLA-DR3 Antigen/*genetics
MH  - HLA-DR4 Antigen/*genetics
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunophenotyping
MH  - Infant
MH  - Membrane Proteins/*immunology
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 1
MH  - Protein Tyrosine Phosphatases/*immunology
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 8
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1007/BF02658510 [doi]
PST - ppublish
SO  - Diabetologia. 1996 Oct;39(10):1223-6. doi: 10.1007/BF02658510.