PMID- 8897443
OWN - NLM
STAT- MEDLINE
DCOM- 19970324
LR  - 20190726
IS  - 0028-1298 (Print)
IS  - 0028-1298 (Linking)
VI  - 354
IP  - 4
DP  - 1996 Oct
TI  - Effect of glutamate receptor ligands on mitochondrial membrane potential in rat 
      dissociated cerebellar cells.
PG  - 420-3
AB  - The effect of three different glutamate receptor ligands on mitochondrial 
      membrane potential has been studied in rat pup dissociated cerebellar cells by 
      measuring rhodamine 123 fluorescence. L-glutamate, NMDA (N-methyl-D-aspartate) 
      and kainate (from 10(-8) to 10(-3) M) decreased in a concentration-dependent 
      manner the mitochondrial membrane potential with EC50 values of 6.7 +/- 1.7, 3.8 
      +/- 0.5, and 37.4 +/- 14 microM, respectively. Dizocilpine ((+)MK 801) was able 
      to inhibit the NMDA- and L-glutamate-induced decrease in rhodamine 123 
      fluorescence, while kainate-induced fluorescence-decreases were unaffected. 
      However, 6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX) totally prevented the 
      effect of kainate on mitochondrial membrane potential, but failed to block the 
      L-glutamate effect. It is concluded that, in our cell preparation, L-glutamate 
      exerts its action mainly through NMDA-subtype receptors, and that Ca2+ and Na+ 
      entry through ionotropic glutamate receptors could be responsible for an 
      impairment of mitochondrial membrane potential.
FAU - Sureda, F X
AU  - Sureda FX
AD  - Unitat de Farmacologia i Farmacognosia, Facultat de Farmacia, Universitat de 
      Barcelona, Spain.
FAU - Escubedo, E
AU  - Escubedo E
FAU - Gabriel, C
AU  - Gabriel C
FAU - Camarasa, J
AU  - Camarasa J
FAU - Camins, A
AU  - Camins A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Naunyn Schmiedebergs Arch Pharmacol
JT  - Naunyn-Schmiedeberg's archives of pharmacology
JID - 0326264
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Rhodamines)
RN  - 1N3CZ14C5O (Rhodamine 123)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 9NEZ333N27 (Sodium)
RN  - SIV03811UC (Kainic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Calcium/metabolism
MH  - Cerebellum/cytology/*drug effects/metabolism
MH  - Fluorescent Dyes
MH  - Glutamic Acid/pharmacology
MH  - In Vitro Techniques
MH  - Kainic Acid/pharmacology
MH  - Ligands
MH  - Membrane Potentials/*drug effects
MH  - Mitochondria/*drug effects/metabolism
MH  - N-Methylaspartate/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Glutamate/*metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Rhodamine 123
MH  - Rhodamines
MH  - Sodium/metabolism
MH  - Spectrometry, Fluorescence
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1007/BF00168431 [doi]
PST - ppublish
SO  - Naunyn Schmiedebergs Arch Pharmacol. 1996 Oct;354(4):420-3. doi: 
      10.1007/BF00168431.